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首页> 外文期刊>Journal of Experimental Botany >Na+/H+ exchanger 1 participates in tobacco disease defence against Phytophthora parasitica var. nicotianae by affecting vacuolar pH and priming the antioxidative system
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Na+/H+ exchanger 1 participates in tobacco disease defence against Phytophthora parasitica var. nicotianae by affecting vacuolar pH and priming the antioxidative system

机译：Na + / H +交换子1参与抗寄生疫霉菌的烟草疾病防御。通过影响液泡pH值和引发抗氧化系统来实现烟草

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摘要

Despite the importance of NHX1 (Na+/H+ exchanger 1) in plant salt tolerance, little is known about its other functions. In this study, intriguingly, it was found that NHX1 participated in plant disease defence against Phytophthora parasitica var. nicotianae (Ppn) in Nicotiana benthamiana. NbNHX1 was originally isolated from N. benthamiana, and characterized. The subcellular localization of NbNHX1 with its C-terminus fused with green fluorescent protein indicated that NbNHX1 localized primarily to the tonoplast. Tobacco rattle virus-induced NbNHX1 silencing led to reduced H+ efflux from the vacuole to cytoplasts, and decreased Ppn resistance in N. benthamiana. After attack by Ppn, NbNHX1-silenced plants exhibited impaired ability to scavenge reactive oxidative species (ROS) induced by the pathogen. Pea early browning virus-mediated ectopic expression of SeNHX1 (from Salicornia europaea) or AtNHX1 (from Arabidopsis thaliana) both conferred enhanced Ppn resistance to N. benthamiana, with a lower H2O2 concentration after Ppn inoculation. Further investigation of the role of NHX1 demonstrated that transient overexpression of NbNHX1 improved the vacuolar pH and cellular ROS level in N. benthamiana, which was coupled with an enlarged NAD(P) (H) pool and higher expression of ROS-responsive genes. In contrast, NbNHX1 silencing led to a lower pH in the vacuole and a lower cellular ROS level in N. benthamiana, which was coupled with a decreased NAD(P) (H) pool and decreased expression of ROS-responsive genes. These results suggest that NHX1 is involved in plant disease defence; and regulation of vacuolar pH by NHX1, affecting the cellular oxidation state, primes the antioxidative system which is associated with Ppn resistance in tobacco.
机译：尽管NHX1（Na + / H +交换剂1）在植物耐盐性中很重要，但对其其他功能知之甚少。有趣的是，在这项研究中，NHX1参与了抗寄生疫霉菌的植物病害防御。 nicotiana benthamiana的nicotianae（Ppn）。 NbNHX1最初从本氏烟草中分离出来，并进行了表征。 NbNHX1的亚细胞定位及其C末端融合有绿色荧光蛋白，表明NbNHX1主要定位在液泡膜中。烟草摇铃病毒诱导的NbNHX1沉默导致从液泡到细胞质的H +流出减少，并降低了本氏烟草对Ppn的抗性。在受到Ppn攻击后，NbNHX1沉默的植物清除病原体诱导的反应性氧化物质（ROS）的能力受损。豌豆早褐变病毒介导的SeNHX1（来自Salicornia europaea）或AtNHX1（来自拟南芥）的异位表达均增强了对本氏猪笼草的Ppn抗性，接种Ppn后其H2O2浓度较低。 NHX1作用的进一步研究表明，NbNHX1的瞬时过表达改善了本氏烟草的液泡pH和细胞ROS水平，这与扩大的NAD（P）（H）库和更高的ROS反应基因表达相结合。相比之下，NbNHX1沉默导致液泡中较低的pH值和本氏烟草中较低的细胞ROS水平，这与NAD（P）（H）库减少和ROS响应基因表达降低有关。这些结果表明，NHX1参与了植物防病; NHX1对液泡pH的调节，影响细胞的氧化状态，引发了与烟草中Ppn耐药性相关的抗氧化系统。

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