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首页> 外文期刊>Journal of feline medicine and surgery >Diagnosis of feline haemoplasma infection in Australian cats using a real-time PCR assay.
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Diagnosis of feline haemoplasma infection in Australian cats using a real-time PCR assay.

机译：使用实时PCR分析法诊断澳大利亚猫的血友病。

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摘要

A total of 147 cats from the Sydney area of Australia that had blood samples submitted to veterinary laboratories were tested using a real-time polymerase chain reaction (PCR) assay able to detect and distinguish the two feline haemoplasma species. This sample number included two cats diagnosed with feline haemoplasma infection by routine blood smear examination. Statistical analysis was performed to evaluate associations between haemoplasma infection, age, sex, breed, haematocrit (HCT) values and anaemia status. One hundred and six cats (72.1%) were negative. Thirty-four cats (23.1%) were positive for 'Candidatus M. haemominutum', six cats (4.1%) were positive for M. haemofelis and one cat (0.7%) was positive for both species. Older, male, non-pedigree cats, with lower HCT values were more likely to be infected with 'Candidatus M. haemominutum'. Significant inverse correlation was found between the amount of M. haemofelis DNA present in the blood and the HCT value. This report documents the existence of, and prevalence of, both haemoplasma species in a sample of cats in Australia and is the first to use quantitative real-time PCR in a prevalence study for haemoplasma infection.
机译：使用实时聚合酶链反应（PCR）检测试剂盒对澳大利亚悉尼地区的147只猫的血样提交兽医实验室进行了测试，该试剂盒能够检测和区分两种猫科血肿。该样本数量包括两只通过常规血液涂片检查诊断为猫血友病感染的猫。进行统计分析以评估血肿感染，年龄，性别，品种，血细胞比容（HCT）值与贫血状况之间的关联。一百零六只猫（72.1％）为阴性。两种物种的“假单胞菌”阳性的有34只猫（23.1％），血肠分枝杆菌的阳性的有6只猫（4.1％），而两个物种的阳性有1只猫（0.7％）。 HCT值较低的年长，雄性，非谱系猫更容易感染'Candidatus M. haemominutum'。发现血液中存在的嗜血分支杆菌DNA数量与HCT值之间存在显着的反相关关系。该报告记录了澳大利亚的猫样本中两种血友病物种的存在和流行情况，并且是在血友病感染流行研究中首次使用定量实时PCR的方法。

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