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首页> 外文期刊>Journal of Fish Biology >Choice of microsatellite markers for identifying homozygosity of mitotic gynogenetic diploids in Japanese flounder Paralichthys olivaceus
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Choice of microsatellite markers for identifying homozygosity of mitotic gynogenetic diploids in Japanese flounder Paralichthys olivaceus

机译：微卫星标记的选择，以鉴定日本比目鱼Paralichthys olivaceus有丝分裂的雌核发育二倍体的纯合性

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摘要

A set of 72 microsatellite markers distributed evenly among 24 linkage groups were selected from the published genetic linkage maps of Japanese flounder Paralichthys olivaceus. In two normal diploid full-sib families, the test for Mendelian inheritance showed that genotypic segregation deviations were not significant at all analysed loci. To estimate microsatellite-centromere map distances, four meiotic gynogenetic diploid lines were produced by the activation of eggs using UV irradiated sperm of red seabream Pagrus major and cold-shock treatment to block the extrusion of the second polar body. Under the assumption of complete interference, 21 markers were located in the centromeric region, 39 in the telomeric region and the rest in the intermediate region of linkage groups. A total of 192 mitotic gynogenetic diploids from one spawn were identified by these markers. Genotype analysis showed that the number of homozygous individuals decreased as microsatellite-centromere map distance increased on each linkage group.
机译：从公布的日本比目鱼比目鱼的遗传连锁图谱中选择了24个连锁组中均匀分布的72个微卫星标记。在两个正常的二倍体全同胞家族中，孟德尔遗传测试表明，在所有分析的基因座上，基因型分离差异均不显着。为了估计微卫星-着丝粒图的距离，通过使用红鲷鱼大虫的紫外线辐射精子和冷激处理来阻止第二极体的挤出，通过激活卵来产生四个减数分裂的雌核发育的二倍体系。在完全干扰的假设下，在着丝粒区域中有21个标记，在端粒区域中有39个标记，在连接基团的中间区域中有其余标记。通过这些标记鉴定出来自一个产卵的总共192个有丝分裂的雌激素发育二倍体。基因型分析表明，随着每个连锁组微卫星-着丝粒图距离的增加，纯合子的数量减少。

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