...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Fish Biology >Rapid, economical single-nucleotide polymorphism and microsatellite discovery based on de novo assembly of a reduced representation genome in a non-model organism: a case study of Atlantic cod Gadus morhua
【24h】

Rapid, economical single-nucleotide polymorphism and microsatellite discovery based on de novo assembly of a reduced representation genome in a non-model organism: a case study of Atlantic cod Gadus morhua

机译：快速，经济的单核苷酸多态性和微卫星发现，基于从头开始装配的非模式生物中减少的代表性基因组：大西洋鳕鱼加多斯·莫尔瓦的案例研究

获取原文
获取原文并翻译 | 示例
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

By combining next-generation sequencing technology (454) and reduced representation library (RRL) construction, the rapid and economical isolation of over 25 000 potential single-nucleotide polymorphisms (SNP) and>6000 putative microsatellite loci from c. 2% of the genome of the non-model teleost, Atlantic cod Gadus morhua from the Celtic Sea, south of Ireland, was demonstrated. A small-scale validation of markers indicated that 80% (11 of 14) of SNP loci and 40% (6 of 15) of the microsatellite loci could be amplified and showed variability. The results clearly show that small-scale next-generation sequencing of RRL genomes is an economical and rapid approach for simultaneous SNP and microsatellite discovery that is applicable to any species. The low cost and relatively small investment in time allows for positive exploitation of ascertainment bias to design markers applicable to specific populations and study questions.
机译：通过结合下一代测序技术（454）和简化表示库（RRL）的构建，可以从c中快速经济地分离出超过25 000个潜在的单核苷酸多态性（SNP）和> 6000个推定的微卫星基因座。展示了来自爱尔兰南部凯尔特海的非模式硬骨鱼类（鳕鱼Gadus morhua）的2％基因组。标记物的小规模验证表明，可以扩增80％（14个中的11个）的SNP位点和40％（15个中的6个）的微卫星位点，并显示出变异性。结果清楚地表明，RRL基因组的小规模下一代测序是一种经济，快速的方法，可同时发现SNP和微卫星，适用于任何物种。低成本和相对较少的时间投入可以肯定地利用确定性偏倚来设计适用于特定人群和研究问题的标记。

著录项

相似文献

  • 外文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号