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首页> 外文期刊>Journal of experimental zoology, Part B. Molecular and developmental evolution >Comparative Cis-regulatory Analyses Identify New Elements of the Mouse Hoxc8 Early Enhancer
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Comparative Cis-regulatory Analyses Identify New Elements of the Mouse Hoxc8 Early Enhancer

机译：比较顺式调节分析，识别小鼠Hoxc8早期增强子的新元素。

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The Hoxc8 early enhancer is a 200 bp region that controls the early phase of Hoxc8 expression during mouse embryonic development. This enhancer defines the domain of Hoxc8 expression in the neural tube and mesoderm of the posterior regions of the developing embryo. Five distinct cis-acting elements, A-E, were previously shown to govern early phase Hoxc8 expression. Significant divergence between mammalian and fish Hoxc8 early enhancer sequences and activities suggested additional cis-acting elements. Here we describe four additional cis-acting elements (F-I) within the 200 bp Hoxc8 early enhancer region identified by comparative regulatory analysis and transgene-mutation studies. These elements affect posterior neural tube and mesoderm expression of the reporter gene, either singly or in combination. Surprisingly, these new elements are missing from the zebrafish and Fugu Hoxc8 early enhancer sequences. Considering that fish enhancers direct robust reporter expression in transgenic mouse embryos, it is tempting to postulate that fish and mammalian Hoxc8 early enhancers utilize different sets of elements to direct Hoxc8 early expression. These observations reveal a remarkable plasticity in the Hoxc8 early enhancer, suggesting different modes of initiation and establishment of Hoxc8 expression in different species. We postulate that extensive restructuring and remodeling of Hox cis-regulatory regions occurring in different taxa lead to relatively different Hox expression patterns, which in turn may act as a driving force in generating diverse axial morphologies.
机译：Hoxc8早期增强子是一个200 bp的区域，可控制小鼠胚胎发育过程中Hoxc8表达的早期阶段。该增强子定义了Hoxc8在发育中的胚胎后部区域的神经管和中胚层中的表达域。先前显示五个不同的顺式作用元件A-E可以控制早期Hoxc8表达。哺乳动物和鱼类Hoxc8早期增强子序列和活性之间的显着差异提示了其他顺式作用元件。在这里，我们描述了通过比较调控分析和转基因突变研究确定的200 bp Hoxc8早期增强子区域内的四个其他顺式作用元件（F-I）。这些元素单独或组合影响报告基因的后神经管和中胚层表达。令人惊讶的是，斑马鱼和Fugu Hoxc8早期增强子序列中缺少这些新元素。考虑到鱼类增强子指导转基因小鼠胚胎中强劲的报告基因表达，因此很容易假设鱼类和哺乳动物的Hoxc8早期增强子利用不同的元件来指导Hoxc8早期表达。这些观察结果揭示了Hoxc8早期增强子中的显着可塑性，表明不同物种中Hoxc8表达的起始和建立模式不同。我们假设发生在不同分类群中的Hox顺式调控区域的广泛重组和重塑导致相对不同的Hox表达模式，这反过来可能会成为产生各种轴向形态的驱动力。

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