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首页> 外文期刊>Journal of Fluorescence >Synthesis of AS1411-Aptamer-Conjugated CdTe Quantum Dots with High Fluorescence Strength for Probe Labeling Tumor Cells
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Synthesis of AS1411-Aptamer-Conjugated CdTe Quantum Dots with High Fluorescence Strength for Probe Labeling Tumor Cells

机译：具有高荧光强度的AS1411-适体共轭的CdTe量子点的合成，用于探针标记肿瘤细胞

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In this paper, we report microwave-assisted, onestage synthesis of high-quality functionalized water-soluble cadmium telluride (CdTe) quantum dots (QDs). By selecting sodium tellurite as the Te source, cadmium chloride as the Cd source, mercaptosuccinic acid (MSA) as the capping agent, and a borate-acetic acid buffer solution with a pH range of 5- 8, CdTe nanocrystals with four colors (blue to orange) were conveniently prepared at 100 °C under microwave irradiation in less than one hour (reaction time:10-60 min). The influence of parameters such as the pH, Cd:Te molar ratio, and reaction time on the emission range and quantum yield percentage (QY%) was investigated. The structures and compositions of the prepared CdTe QDs were characterized by transmission electron microscopy, energy-dispersive X-ray spectroscopy, selective area electron diffraction, and X-ray powder diffraction experiments. The formation mechanism of the QDs is discussed in this paper. Furthermore, AS1141-aptamer-conjugated CdTe QDs in the U87MG glioblastoma cell line were assessed with a fluorescence microscope. The obtained results showed that the best conditions for obtaining a high QY of approximately 87% are a pH of 6, a Cd:Te molar ratio of 5:1, and a 30-min reaction time at 100 °C under microwave irradiation. The results showed that AS1141-aptamer-conjugated CdTe QDs could enter tumor cells efficiently. It could be concluded that a facile high-fluorescencestrength QD conjugated with a DNA aptamer, AS1411, which can recognize the extracellular matrix protein nucleolin, can specifically target U87MG human glioblastoma cells. The qualified AS1411-aptamer-conjugated QDs prepared in this study showed excellent capabilities as nanoprobes for cancer targeting and molecular imaging.
机译：在本文中，我们报道了微波辅助一步法合成的高质量功能化水溶性碲化镉（CdTe）量子点（QDs）。通过选择亚碲酸钠作为Te来源，氯化镉作为Cd来源，巯基琥珀酸（MSA）作为封端剂以及pH范围为5至8的硼酸-乙酸缓冲溶液，CdTe纳米晶体具有四种颜色（蓝色）。通常在不到一小时的时间内在微波辐射下于100°C（反应时间：10-60分钟）制备三氟甲磺酸（橙）。研究了pH，Cd：Te摩尔比，反应时间等参数对发光范围和量子产率百分比（QY％）的影响。通过透射电子显微镜，能量色散X射线光谱，选择性区域电子衍射和X射线粉末衍射实验对制备的CdTe量子点的结构和组成进行了表征。本文讨论了量子点的形成机理。此外，用荧光显微镜评估了U87MG胶质母细胞瘤细胞系中与AS1141-适体结合的CdTe QD。获得的结果表明，获得约87％的高QY的最佳条件是pH为6，Cd：Te摩尔比为5：1以及在微波辐射下于100°C的30分钟反应时间。结果表明，结合了AS1141-适体的CdTe QDs可以有效进入肿瘤细胞。可以得出结论，与DNA适体AS1411偶联的轻质高荧光强度QD可以识别细胞外基质蛋白核仁素，可以特异性靶向U87MG人胶质母细胞瘤细胞。在这项研究中制备的合格的AS1411-适体共轭QD显示出出色的能力，可作为靶向癌症和分子成像的纳米探针。

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