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Environmental Staphylococcus aureus contamination in a Tunisian hospital

机译:突尼斯一家医院的环境金黄色葡萄球菌污染

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摘要

One hundred hospital environment samples were obtained in 2012 in a Tunisian hospital and tested for Staphylococcus aureus recovery. Antimicrobial resistance profile and virulence gene content were determined. Multilocus-sequence-typing (MLST), spa-typing, agr-typing and SmaI-pulsed-field gel electrophoresis (PFGE) were performed. Two methicillin-resistant S. aureus (MRSA) isolates typed as: ST247-t052-SCCmecI-agrI were recovered from the intensive care unit (ICU). Ten samples contained methicillin-susceptible S. aureus (MSSA) and these samples were collected in different services, highlighting the presence of the tst gene encoding the toxic shock syndrome toxin as well as the luk ED, hla, hlb, hld and hlg(v) virulence genes in some of the isolates. In conclusion, we have shown that the hospital environment could be a reservoir contributing to dissemination of virulent S. aureus and MRSA.
机译:2012年在突尼斯一家医院获得了100个医院环境样本,并对其金黄色葡萄球菌的恢复进行了测试。测定抗菌素耐药性和毒力基因含量。进行多基因座序列分型(MLST),spa分型,农业分型和SmaI脉冲场凝胶电泳(PFGE)。从重症监护病房(ICU)中回收了两个耐甲氧西林的金黄色葡萄球菌(MRSA)分离株:ST247-t052-SCCmecI-agrI。十个样本中含有对甲氧西林敏感的金黄色葡萄球菌(MSSA),这些样本在不同的服务机构中收集,突显了编码毒性休克综合症毒素的tst基因以及luk ED,hla,hlb,hld和hlg(v)的存在)某些分离物中的毒力基因。总之,我们已经表明,医院环境可能是有助于传播有毒金黄色葡萄球菌和MRSA的蓄水池。

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