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首页> 外文期刊>Journal of general plant pathology >Identification and characterization of lisianthus necrotic ringspot virus, a novel distinct Tospovirus species causing necrotic disease of lisianthus (Eustoma grandiflorum).
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Identification and characterization of lisianthus necrotic ringspot virus, a novel distinct Tospovirus species causing necrotic disease of lisianthus (Eustoma grandiflorum).

机译：桔梗坏死环斑病毒的鉴定和鉴定，桔梗坏死环斑病毒是一种引起桔梗坏死病的新型独特的Tospovirus物种。

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摘要

In 2007, lisianthus (Eustoma grandiflorum) plants with necrotic ringspots on the leaves were found in Kochi Prefecture, Japan. Tospovirus-like spherical enveloped particles with ca. 160 nm in diameter were observed with electron microscopy. The complete nucleotide sequence of the S RNA segment of the virus was determined, and phylogenetic analysis using deduced amino acid sequences of the nucleocapsid protein and the nonstructural S protein indicated that the virus is phylogenetically distinct from any known tospovirus species. The results suggest that the virus is a new member of the genus Tospovirus, in the family Bunyaviridae. The virus is the fifth distinct tospovirus occurring naturally in lisianthus in Japan. The necrotic symptoms were reproduced on lisianthus seedlings after mechanical inoculation. The host range of the virus isolate on several test plants was also examined.
机译：2007年，在日本高知县发现了叶片上有坏死环斑的桔梗（Eustoma grandiflorum）植物。具Tospovirus状的球形包膜颗粒，约含。用电子显微镜观察到直径为160nm。确定了病毒S RNA片段的完整核苷酸序列，并使用推论的核衣壳蛋白和非结构性S蛋白氨基酸序列进行了系统进化分析，表明该病毒在系统发育上与任何已知的脊突病毒种类不同。结果表明该病毒是Bunyaviridae家族中Tospovirus属的新成员。该病毒是日本桔梗中天然存在的第五种独特的弓形病毒。机械接种后，坏死症状在桔梗幼苗上重现。还检查了几种测试植物上病毒分离株的宿主范围。

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