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首页> 外文期刊>Journal of genetics >Physical localization of NORs and ITS length variants in old Portuguese durum wheat cultivars
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Physical localization of NORs and ITS length variants in old Portuguese durum wheat cultivars

机译：葡萄牙硬粒小麦品种NORs及其ITS长度变异体的物理定位

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摘要

The variation at the internal transcribed spacer (ITS) region of the ribosomal DNA has been correlated with the number of nucleolar organizer regions (NORs) in some plant species. Besides, the number of NORs might influence the rate of homogenization of the rDNA repeats. In recent studies, ITS length variants were detected in bread wheat cultivars but no reports about their presence in durum wheat were found. In the present study, we localized and identified the NORs of 51 old Portuguese durum wheat cultivars by using sequential silver staining and fluorescence in situ hybridization performed with the pTa71 rDNA probe. We also detected ITS length variants by PCR-RFLP. No variation at the number of Ag-NORs per metaphase was found among the 51 durum wheat cultivars, but the PCR-RFLP technique carried out with the restriction enzyme HpaII, allowed the detection of ITS length variants among them. The molecular data was used in order to establish the genetic relationships among cultivars and botanical varieties of durum wheat. The knowledge of this feature could be useful for future design of breeding strategies, involving this collection that constitutes an excellent repository of germplasm in Portugal.
机译：核糖体DNA内部转录间隔区（ITS）区域的变异与某些植物物种中核仁组织区（NORs）的数量相关。此外，NOR的数量可能会影响rDNA重复序列的均质化速率。在最近的研究中，在面包小麦品种中检测到ITS长度变异，但未发现有关在硬粒小麦中存在的报道。在本研究中，我们通过使用pTa71 rDNA探针进行的连续银染和荧光原位杂交，对51个老葡萄牙硬质小麦品种的NORs进行了定位和鉴定。我们还通过PCR-RFLP检测到ITS长度变异。在51个硬粒小麦品种中，每个中期的Ag-NORs数量均未发现变化，但是使用限制酶HpaII进行的PCR-RFLP技术可以检测其中的ITS长度变异。使用分子数据来建立硬粒小麦品种和植物品种之间的遗传关系。对该特征的了解可能对将来的育种策略设计有用，涉及构成葡萄牙优良种质资源库的这一收藏。

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