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首页> 外文期刊>Journal of genetics >Characterization of a genome-specific Gypsy-like retrotransposon sequence and development of a molecular marker specific for Dasypyrum villosum (L.)
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Characterization of a genome-specific Gypsy-like retrotransposon sequence and development of a molecular marker specific for Dasypyrum villosum (L.)

机译：基因组特异的吉普赛样反转录转座子序列的表征和绒毛虫特有的分子标记的发展

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As an important gene resource for wheat improvement, genetic materials of Dasypyrum villosum (L.) (2m = 14, VV) have been introduced into wheat genome. Isolation of D. villosum specific DNA sequences, especially the genomewide interspersed repetitive sequences, is an effective way to trace D. villosum chromatin in wheat genetic background during breeding programmes. In this study, a Gypsy-like retrotransposon sequence was isolated by random amplified polymorphic DNA (RAPD), designated as CI-10. Fluorescence in situ hybridization (FISH) revealed that Cl-10 could be specifically hybridized with all D. villosum chromosome arms of Triticum durum - D. villosum amphiploids, as well as segments of D. villosum chromosomes in wheat -D. villosum translocation lines, suggesting that Cl-10 might be a D. villosum specific repetitive sequence, which could be used to distinguish V genome from A, B and D genomes of wheat. Based on sequence of Cl-10, one pair of PCR primers Dvl (Dvl-R and Dvl-F) was designed and could efficiently and specifically amplify the target from five accessions of A villosum, five accessions of T. durum - D. villosum amphiploids, seven accessions of wheat - D. villosum additional lines, and three wheat lines containing D. villosum chromatins. Therefore, it could be used as a sequence-characterized amplified region (SCAR) marker to effectively trace the introgression of D. villosum chromatin in the wheat genome, and consequently will facilitate the utilization of useful genes of D. villosum in wheat improvement.
机译：稻瘟病菌（Dasypyrum villosum（L.）（2m = 14，VV）的遗传材料已作为小麦改良的重要基因资源，已被引入小麦基因组。分离绒毛衣藻特异性DNA序列，尤其是全基因组穿插的重复序列，是在育种程序中追踪小麦遗传背景中绒毛衣藻染色质的有效方法。在这项研究中，通过命名为CI-10的随机扩增多态性DNA（RAPD）分离了吉普赛样逆转录转座子序列。荧光原位杂交（FISH）表明，Cl-10可以与硬质小麦的所有D. villosum染色体臂-D. villosum二倍体，以及小麦-D中的D. villosum染色体片段特异性杂交。绒毛易位系，提示Cl-10可能是绒毛木霉特异的重复序列，可用于区分小麦的V基因组与A，B和D基因组。基于Cl-10的序列，设计了一对PCR引物Dvl（Dv1-R和Dv1-F），并且可以从五个绒毛的A种，杜氏杜鹃-绒毛的五个种中有效和特异性地扩增靶标。二倍体，小麦的七个种质-D. villosum附加品系和三个包含D. villosum染色质的小麦品系。因此，它可以用作序列特征扩增区（SCAR）标记，以有效追踪小麦基因组中的毛纺锤菌染色质渗入，因此将促进毛纺锤子有用基因在小麦改良中的利用。

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