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首页> 外文期刊>Journal of general plant pathology >Selection and discrimination of an attenuated strain of Chinese yam necrotic mosaic virus for cross-protection
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Selection and discrimination of an attenuated strain of Chinese yam necrotic mosaic virus for cross-protection

机译:交叉保护的山药坏死花叶病毒减毒株的选择与鉴别

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摘要

An attenuated strain of Chinese yam necrotic mosaic virus (CYNMV), designated KM3, was selected from among CYNMV field isolates. KM3 causes a mild mosaic on leaves of Chinese yam (Dioscorea opposita) cv. Nagaimo during the early stages of growth, andthe severity of the symptoms is reduced with growth of the plants. In crossprotection tests using aphids to transmit the virus, Nagaimo plants that had been infected with KM3 were protected from infection with CYNMV severe strains YS117 and IW5. A reverse transcription-polymerase chain reactionestriction fragment length polymorphism (RT-PCR-RFLP) method was developed to discriminate KM3 from other isolates in the field.
机译:从CYNMV田间分离株中选择了减毒株山药坏死花叶病毒(CYNMV),命名为KM3。 KM3在山药(c)的叶片上产生轻微的花叶。 Nagaimo在生长的早期阶段,症状的严重程度随植物的生长而降低。在使用蚜虫传播病毒的交叉保护测试中,已保护已感染KM3的长门植物免受CYNMV严重株YS117和IW5的感染。逆转录聚合酶链反应限制性片段长度多态性(RT-PCR-RFLP)方法被开发出来,以区分KM3与该领域中的其他分离株。

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