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首页> 外文期刊>Journal of genetics >Physical location of SSR regions and cytogenetic instabilities in Pinus sylvestris chromosomes revealed by ND-FISH
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Physical location of SSR regions and cytogenetic instabilities in Pinus sylvestris chromosomes revealed by ND-FISH

机译：ND-FISH揭示樟子松染色体SSR区域的物理定位和细胞遗传学的不稳定性

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摘要

Portugal constitutes the southwestern end limit of the distribution area of Scots pine in the world. The population of ‘Ribeira das Negras’ (‘Serra do Gerês’; NW Portugal) has been considered potentially native and peripheral. This species has 24 chromosomes, hardly distinguishable because of their similar size and shape. Cytogenetic studies are scarce and instabilities were previously reported in peripheral Scots pine populations. Here, we intended to cytogenetically characterize individuals from ‘Ribeira das Negras’ using 14 simple sequence repeats (SSRs) and 45S rDNA sequence (pTa71) as probes, by nondenaturing fluorescence in situ hybridization (ND-FISH) and FISH, respectively. Eight SSRs [(AC)_(10); (AG)_(10); (AG)_(12); (AAG)_5; (AAC)_5; (GATA)_4; (GACA)_5 and (GGAT)_4] and pTa71 showed hybridization. The (AG)_(10) probe hybridized on all chromosomes and an ideogram was constructed. Each metaphase cell presented cytogenetic instabilities corroborating ‘Ribeira das Negras’ as a peripheral population. As far as we know, this is the first cytogenetic study in Scots pine using SSRs in FISH experiments.
机译：葡萄牙是世界上苏格兰松树分布地区的西南端。 “ Ribeira das Negras”（“ Serra doGerês”;葡萄牙西北）的人口被认为是潜在的本地人和边缘人。该物种有24条染色体，由于它们的大小和形状相似而难以区分。细胞遗传学研究很少，以前在外围苏格兰松树种群中也报道过不稳定。在这里，我们打算分别通过非变性荧光原位杂交（ND-FISH）和FISH，使用14个简单序列重复（SSR）和45S rDNA序列（pTa71）作为探针，对'Ribeira das Negras'的个体进行细胞遗传学表征。八个SSR [（AC）_（10）; （AG）_（10）; （AG）_（12）; （AAG）_5; （AAC）_5; （GATA）_4; （GACA）_5和（GGAT）_4]与pTa71显示杂交。 （AG）_（10）探针在所有染色体上杂交并构建了表意图。每个中期细胞均表现出细胞遗传学上的不稳定性，从而证实了“ Ribeira das Negras”为外围种群。据我们所知，这是在FISH实验中使用SSR在苏格兰松树中进行的首次细胞遗传学研究。

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