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首页> 外文期刊>Journal of genetics >Construction of intersubspecific molecular genetic map of lentil based on ISSR, RAPD and SSR markers
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Construction of intersubspecific molecular genetic map of lentil based on ISSR, RAPD and SSR markers

机译：基于ISSR，RAPD和SSR标记的扁豆亚种间分子遗传图谱的构建

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Lentil (Lens culinaris ssp. culinaris), is a self-pollinating diploid (2n = 2x = 14), cool-season legume crop and is consumed worldwide as a rich source of protein (~24. 0%), largely in vegetarian diets. Here we report development of a genetic linkage map of Lens using 114 F_2 plants derived from the intersubspecific cross between L 830 and ILWL 77. RAPD (random amplified polymorphic DNA) primers revealed more polymorphism than ISSR (intersimple sequence repeat) and SSR (simple sequence repeat) markers. The highest proportion (30. 72%) of segregation distortion was observed in RAPD markers. Of the 235 markers (34 SSR, 9 ISSR and 192 RAPD) used in the mapping study, 199 (28 SSRs, 9 ISSRs and 162 RAPDs) were mapped into 11 linkage groups (LGs), varying between 17. 3 and 433. 8 cM and covering 3843. 4 cM, with an average marker spacing of 19. 3 cM. Linkage analysis revealed nine major groups with 15 or more markers each and two small LGs with two markers each, and 36 unlinked markers. The study reported assigning of 11 new SSRs on the linkage map. Of the 66 markers with aberrant segregation, 14 were unlinked and the remaining 52 were mapped. ISSR and RAPD markers were found to be useful in map construction and saturation. The current map represents maximum coverage of lentil genome and could be used for identification of QTL regions linked to agronomic traits, and for marker-assisted selection in lentil.
机译：扁豆（Lens culinaris ssp。culinaris）是一种自花授粉的二倍体（2n = 2x = 14），是凉爽的豆类作物，在世界范围内以丰富的蛋白质来源（〜24。0％）消费，主要用于素食。 。在这里，我们报道了使用来自L 830和ILWL 77之间的亚种间杂交的114株F_2植物开发的Lens的遗传连锁图谱。RAPD（随机扩增的多态DNA）引物比ISSR（简单重复序列）和SSR（简单序列）具有更多的多态性。重复）标记。在RAPD标记中观察到最高比例的偏析（30. 72％）。在作图研究中使用的235个标记（34个SSR，9个ISSR和192 RAPD）中，有199个（28个SSR，9个ISSR和162个RAPD）被映射到11个连锁组（LG）中，在17. 3和433之间变化。8 cM，覆盖3843. 4 cM，平均标记间距为19。3cM。连锁分析显示九个主要组，每个组具有15个或更多个标记，两个小LG各自具有两个标记，以及36个未链接的标记。该研究报告在连锁图上分配了11个新的SSR。在具有异常隔离的66个标记中，有14个未链接，其余52个已作图。发现ISSR和RAPD标记在地图构建和饱和度方面很有用。当前的图谱代表了扁豆基因组的最大覆盖范围，可用于鉴定与农艺性状相关的QTL区域，并用于扁豆中的标记辅助选择。

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