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Genetic analysis of fertility restoration under CGMS system in rice (Oryza sativa L.) using three-way test-cross method

机译:CGMS系统水稻三倍回交育性恢复的遗传分析

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We studied the genetics of fertility restoration by producing three-way test cross (TWTC) hybrids involved different combinations of restorers, maintainers and partial restorers of rice. Pollen and spikelet fertility of 16 TWTC hybrids were studied. Six TWTC involving restorer/restorer combinations as male parents produced progenies with fertility levels ranging from complete to zero. No specific ratio of segregation was observed. The crosses involving maintainer/maintainer combinations as male parents showed fully fertile and partial fertile/sterile plants in their progenies. These could be due to nonallelic gene interactions for fertility restoration between the two restorer or maintainer parents, or due to the influence of some modifying genes in the nuclear genome. TWTC involving partial restorer / restorer and partial restorer/partial restorer as the male parents also produced fully fertile and partial fertile/sterile plants suggesting the complex genetics of fertility restoration in rice. There were no previous results depicting the complementation effects of maintainers for fertility restoration.
机译:我们通过生产涉及水稻恢复子,保持子和部分恢复子的不同组合的三效杂交(TWTC)杂交种研究了生育力恢复的遗传学。研究了16个TWTC杂种的花粉和小穗育性。六个TWTC涉及恢复系/恢复系的组合,因为雄性亲本产生的后代的生育率范围从完全到零。没有观察到特定的分离比例。涉及作为父母的维持者/维持者组合的杂交在其后代中显示完全可育和部分可育/不育植物。这些可能是由于两个恢复者或维持者亲本之间恢复生育力的非等位基因相互作用,或归因于核基因组中某些修饰基因的影响。 TWTC涉及部分恢复体/恢复体和部分恢复体/部分恢复体,因为雄性亲本也产生了完全可育的和部分可育/不育的植物,这表明水稻育性恢复的复杂遗传学。以前没有结果描述维持者对生育力恢复的补充作用。

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