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Genetic diversity among old Portuguese bread wheat cultivars and botanical varieties evaluated by ITS rDNA PCR-RFLP markers

机译:ITS rDNA PCR-RFLP标记评估葡萄牙老面包小麦品种和植物品种的遗传多样性

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摘要

In contrast to the highly conserved 18S-5.8S-25S ribosomal RNA (rRNA) genes, the ribosomal DNA (rDNA) spacers, such as the internal transcribed spacer (ITS) and the intergenic spacer (IGS) present high variability and faster rates of evolution (Baldwin et al. 1995; Barker et al. 1988). ITS length variants have been reported for several plant species, including hexaploid wheat (Baldwin et al. 1995; Alvarez and Wendel 2003; Nalini et al. 2007; Saini et al. 2008). The ITS polymorphism might occur at the genus, species or individual levels, making it useful for phylogenetic, evolutionary and biogeographical studies.
机译:与高度保守的18S-5.8S-25S核糖体RNA(rRNA)基因相反,核糖体DNA(rDNA)间隔子,例如内部转录间隔子(ITS)和基因间间隔子(IGS),具有很高的变异性和更快的转录速度。进化(Baldwin等,1995; Barker等,1988)。已经报道了几种植物物种的ITS长度变异,包括六倍体小麦(Baldwin等,1995; Alvarez和Wendel,2003; Nalini等,2007; Saini等,2008)。 ITS多态性可能发生在属,种或个体水平上,使其可用于系统发育,进化和生物地理研究。

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