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Genetic characterization of Perna viridis L. in peninsular Malaysia using microsatellite markers

机译:利用微卫星标记对马来西亚半岛Perna viridis L.进行遗传鉴定

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摘要

A total of 19 polymorphic microsatellite loci were used to analyse levels of genetic variation for 10 populations of Perna viridis L. collected from all over peninsular Malaysia. The populations involved in this study included Pulau Aman in Penang, Tanjung Rhu in Kedah, Bagan Tiang in Perak, Pulau Ketam in Selangor, Muar, Parit Jawa, Pantai Lido and Kampung Pasir Puteh in Johore, and Kuala Pontian and Nenasi in Pahang state. The number of alleles per locus ranged from two to seven, with an average of 3.1. Heterozygote deficiencies were observed across all the 10 populations. Characterization of the populations revealed that local populations of P. viridis in peninsular Malaysia were genetically similar enough to be used as a biomonitoring agent for heavy metal contamination in the Straits of Malacca. Cluster analysis grouped the P. viridis populations according to their geographical distributions with the exception of Parit Jawa. The analysis also revealed that P. viridis from the northern parts of peninsular Malaysia were found to be the most distant populations among the populations of mussels investigated and P. viridis from the eastern part of peninsular Malaysia were closer to the central and southern populations than to the northern populations.
机译:总共使用了19个多态性微卫星基因座来分析从马来西亚半岛各地收集的10个Perna viridis L.种群的遗传变异水平。参与这项研究的人口包括槟城的阿曼岛,吉打州的丹戎R,霹雳州的蒲甘天,雪兰莪的普吉岛,麻坡,柔佛州的Parit Jawa,Pantai Lido和Kampung Pasir Puteh,以及彭亨州的瓜拉庞天和Nenasi。每个基因座的等位基因数量为2到7,平均为3.1。在所有10个人群中都发现了杂合子缺陷。种群特征表明,马来西亚半岛当地的体育假单胞菌在遗传上足够相似,可以用作马六甲海峡中重金属污染的生物监测剂。聚类分析根据绿皮假单胞菌种群的地理分布对它们进行了分组,但Parit Jawa除外。分析还显示,在调查的贻贝种群中,来自马来西亚半岛北部的P. viridis被发现是最远的种群,而来自马来西亚半岛东部的P. viridis则更靠近中部和南部种群。北部人口。

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