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首页> 外文期刊>Journal of genetics >Functional analysis of a Lemna gibba rbcS promoter regulated by abscisic acid and sugar
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Functional analysis of a Lemna gibba rbcS promoter regulated by abscisic acid and sugar

机译：脱落酸和糖调节的Lemna gibba rbcS启动子的功能分析

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摘要

Photosynthesis-associated nuclear genes (PhANGs) are able to respond to multiple environmental and developmental signals, including light, sugar and abscisic acid (ABA). PhANGs have been extensively studied at the level of transcriptional regulation, and several cis-acting elements important for light responsiveness have been identified in their promoter sequences. However, the regulatory elements involved in sugar and ABA regulation of PhANGs have not been completely characterized. A ribulose-1,5-bisphosphate carboxylase small subunit gene (rbcS) promoter (SSU5C promoter) was isolated from duckweed (Lemna gibba). A series of SSU5C promoter 5′ deletion fragments were fused to an intron-gus gene, and transgenic tobacco suspension cell lines were generated. Assay of tobacco suspension cell line harbouring the complete promoter in the fusion construct indicated that SSU5C promoter was negatively regulated by sugar and ABA under the condition of regular photoperiod. 5′ deletion analysis of SSU5C promoter in transgenic tobacco suspension cell lines confirmed that a region between positions -310 and -152 included the ABA-response region, and that sugar-response cis-acting elements might be located in the region between -152 and -117. Taken together, our results confirmed that the cis-regulatory region responsible for repression by ABA and sugar in the SSU5C promoter was located between -310 and -117.
机译：光合作用相关的核基因（PhANGs）能够响应多种环境和发育信号，包括光，糖和脱落酸（ABA）。 PhANGs已在转录调控水平上进行了广泛研究，并且在其启动子序列中已鉴定出对光反应性很重要的几种顺式作用元件。然而，尚未完全表征参与糖和ABA调节PhANGs的调节元件。从浮萍（Lemna gibba）分离了核糖-1,5-双磷酸羧化酶小亚基基因（rbcS）启动子（SSU5C启动子）。将一系列SSU5C启动子5'缺失片段与一个内含子gus基因融合，并产生转基因烟草悬浮细胞系。在融合构建体中具有完整启动子的烟草悬浮细胞系的分析表明，在规则的光周期条件下，SSU5C启动子受到糖和ABA的负调控。转基因烟草悬浮细胞系中SSU5C启动子的5'缺失分析证实，位置-310和-152之间的区域包括ABA反应区域，而糖响应顺式作用元件可能位于-152和-152之间。 -117。两者合计，我们的结果证实，在SSU5C启动子中负责ABA和糖抑制的顺式调控区位于-310和-117之间。

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