Detection of Cronobacter on gluB Gene and Differentiation of Four Cronobacter Species by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism Typing

Ye Yingwang; Ling Na; Han Yongjia; Cao Xiaodong; Wu Qingping

首页> 外文期刊>Journal of Food Safety >Detection of Cronobacter on gluB Gene and Differentiation of Four Cronobacter Species by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism Typing

【24h】

Detection of Cronobacter on gluB Gene and Differentiation of Four Cronobacter Species by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism Typing

机译：聚合酶链反应-限制性片段长度多态性分型检测gluB基因上的克罗诺杆菌并区分四种克罗诺杆菌

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

Cronobacter species are foodborne pathogens associated with neonatal meningitis, sepsis and necrotizing enterocolitis through consumption of contaminated powdered milk. However, there are no specific targets used for differentiating Cronobacter species. The DNA-based assay and polyphasic analysis have facilitated the detection and typing of Cronobacter species in foodborne outbreaks. In this study, polymerase chain reaction (PCR) and dot hybridization for detecting Cronobacter spp. was developed targeting gluB gene, then one PCR-restriction fragment length polymorphism (RFLP) typing assay with TaqI digestion was applied for four species differentiation within Cronobacter. Results indicated that the gluB gene is a specific target for detecting Cronobacter and the PCR-RFLP typing assay could conveniently and rapidly differentiate Cronobactermalonaticus, C.dublinensis, C.sakazakii and C.muytjensii.

机译：克罗诺杆菌是通过食用受污染的奶粉与新生儿脑膜炎，败血症和坏死性小肠结肠炎相关的食源性病原体。但是，没有用于区分Cronobacter物种的特定目标。基于DNA的测定和多相分析促进了食源性暴发中克罗诺杆菌种类的检测和分型。在这项研究中，聚合酶链反应（PCR）和斑点杂交检测克氏杆菌。以gluB基因为靶基因进行了克隆，然后用一种TaqI酶切的PCR酶切限制性片段长度多态性（RFLP）分型法对克罗诺杆菌进行了四种分化。结果表明，gluB基因是检测克罗诺杆菌的特异性靶标，PCR-RFLP分型分析可以方便，快速地区分克罗诺杆菌，杜氏梭菌，阪崎氏梭菌和穆氏梭菌。

著录项

来源
《Journal of Food Safety》 |2015年第3期|共6页
作者
Ye Yingwang; Ling Na; Han Yongjia; Cao Xiaodong; Wu Qingping;
展开▼
作者单位

展开▼
收录信息
原文格式 PDF
正文语种 eng
中图分类食品安全与卫生;
关键词

相似文献

外文文献
中文文献
专利

1. Detection of Cronobacter on gluB Gene and Differentiation of Four Cronobacter Species by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism Typing [J] . Ye Yingwang, Ling Na, Han Yongjia, Journal of Food Safety . 2015,第3期

机译：聚合酶链反应-限制性片段长度多态性分型检测gluB基因上的克罗诺杆菌并区分四种克罗诺杆菌
2. Comparison of 23S polymerase chain reaction-restriction fragment length polymorphism and amplified fragment length polymorphism techniques as typing systems for thermophilic campylobacters [J] . Moreno Y., Ferrus MA., Vanoostende A., FEMS Microbiology Letters . 2002,第1期

机译：比较23S聚合酶链反应限制片段长度多态性和扩增片段长度多态性作为嗜热弯曲杆菌分型系统的技术
3. The identification and differentiation of the Candida parapsilosis complex species by polymerase chain reaction-restriction fragment length polymorphism of the internal transcribed spacer region of the rDNA [J] . Leonardo Silva Barbedo, Mauro de Medeiros Muniz, Rosely Maria Zancopé-Oliveira, Memórias do Instituto Oswaldo Cruz . 2016,第4期

机译：rDNA内部转录间隔区的聚合酶链反应-限制性片段长度多态性鉴定和鉴定假丝酵母复杂种
4. Allele specific polymerase chain reaction for detection of the genetic polymorphism of NMDA receptor [C] . Mohd Nazif Samat Darawi, Ai-Vyrn Chin, Kunanayagam Soraya, 2010 International Conference on Science and Social Research . 2010

机译：等位基因特异性聚合酶链反应检测NMDA受体遗传多态性
5. Egg parasitoids of tabanids in Manitoba: Prevalence, taxonomy, behaviour, and use of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) to establish host-parasite interrelationships. [D] . Iranpour, Mahmood. 2003

机译：曼尼托巴塔宾犬的卵寄生虫：患病率，分类学，行为和使用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）建立宿主-寄生虫相互关系。
6. Comparison of CHROMagar polymerase chain reaction-restriction fragment length polymorphism and polymerase chain reaction-fragment size for the identification of Candida species [O] . Zahra Jafari, Marjan Motamedi, Nilufar Jalalizand, 2017

机译：比较CHROMagar聚合酶链反应限制片段长度多态性和聚合酶链反应片段大小以鉴定念珠菌
7. Polymerase chain reaction-restriction fragment length polymorphism method for differentiation of uropathogenic specific protein gene types [O] . Lai Yun Mei, Zaw Myo Thura, Shamsudin Shamsul Bahari, 2016

机译：聚合酶链反应-限制性片段长度多态性鉴别尿毒症特异性蛋白基因类型的方法

Detection of Cronobacter on gluB Gene and Differentiation of Four Cronobacter Species by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism Typing

摘要

著录项

相似文献

相关主题

期刊订阅