...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biomolecules & therapeutics >Effect of Methotrexate on Collagen-Induced Arthritis Assessed by Micro-Computed Tomography and Histopathological Examination in Female Rats
【24h】

Effect of Methotrexate on Collagen-Induced Arthritis Assessed by Micro-Computed Tomography and Histopathological Examination in Female Rats

机译：甲氨蝶呤对雌性大鼠微计算机断层扫描和组织病理学检查评估的胶原诱导的关节炎的作用

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

We tested the hypothesis that micro-computed tomography (micro-CT) analysis provides a better quantitative readout of the therapeutic potential of methotrexate (MTX) for treating collagen-induced arthritis (CIA) in rats and compared to conventional histopathological examination. Rats were divided into three groups: Group 1 (G1) was treated with 0.9% saline, whereas groups 2 (G2) and 3 (G3) were boosted with type II collagen at days 0 and 7. Following the first collagen immunization, rats in G1 and G2 were treated with 0.9% saline and those in G3 were treated with 1.5 mg/kg MTX from day 14 to 28. All rats were sacrificed on day 28, at which point and all hind knee joints were analyzed by micro-CT and histopathological examination. Micro-CT analyses showed that bone volume and trabecular number were significantly decreased in G2 and G3 compared to G1 (p<0.01), as was percent bone volume (p<0.05 and p<0.01, respectively). However, bone surface/bone volume was significantly increased in G2 and G3 compared to G1 (p<0.05 and p<0.01, respectively). Trabecular separation was significantly increased in G3 compared to G1 (p<0.05). Histopathological examination showed that knee joints of rats in G2 and G3 showed severe joint destruction with inflammatory cell infiltration. However, cartilage destruction was slightly reduced in G3 compared to G2. Taken together, these results suggest that MTX treatment reduced cartilage destruction in rats with CIA, and micro-CT analyses made it possible to quantify arthritic bony lesion.
机译：我们测试了以下假设：微型计算机断层扫描（micro-CT）分析可更好地定量显示甲氨蝶呤（MTX）在治疗大鼠胶原诱导的关节炎（CIA）中的治疗潜力，并与常规组织病理学检查进行比较。将大鼠分为三组：第1组（G1）用0.9％生理盐水处理，而第2组（G2）和第3组（G3）在第0天和第7天用II型胶原加强免疫。从第14天到28天，G1和G2用0.9％的生理盐水处理，而G3中的G1和G2则用1.5 mg / kg MTX处理。在第28天处死所有大鼠，然后通过micro-CT分析所有后膝关节，组织病理学检查。 Micro-CT分析显示，与G1相比，G2和G3中的骨体积和小梁数量显着减少（p <0.01），而骨体积百分数也分别降低（p <0.05和p <0.01）。但是，与G1相比，G2和G3中的骨表面/骨体积显着增加（分别为p <0.05和p <0.01）。与G1相比，G3中的小梁分离明显增加（p <0.05）。组织病理学检查显示，G2和G3大鼠的膝关节出现严重的关节破坏，并伴有炎性细胞浸润。但是，与G2相比，G3中的软骨破坏略有减少。综上所述，这些结果表明，MTX治疗可减少CIA大鼠的软骨破坏，而微CT分析使量化关节炎性骨病变成为可能。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号