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首页> 外文期刊>Journal of hepato-biliary-pancreatic surgery >Analysis of microvessels in pancreatic cancer: by light microscopy, confocal laser scan microscopy, and electron microscopy.
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Analysis of microvessels in pancreatic cancer: by light microscopy, confocal laser scan microscopy, and electron microscopy.

机译：胰腺癌中微血管的分析：通过光学显微镜，共聚焦激光扫描显微镜和电子显微镜。

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BACKGROUND/PURPOSE: The microvessel density (MVD) of most malignant tumors is considered to be strongly related to metastasis and prognosis. Weidner's "hot spot method" for determining MVD is in general use, but it is possible that cells other than endothelial cells will also be stained. In our previous study, no correlations were observed between MVD determined by the "hot spot method" and prognosis/metastasis. But, using the "lumen method," we found a correlation with the number of vessel structures only. In the present study, we analyzed the staining of microvessels in pancreatic cancer, using light microscopy, confocal laser scan microscopy (CLSM), and transmission electron microscopy (TEM). METHODS: Microvessel staining of pancreatic cancer with CD34, factor VIII, and CD45 antibodies was examined in consecutive slices by light microscopy. For CLSM, freshly resected specimens were immunostained with factor VIII and fluorescein isothiocynate. For TEM, specimens were fixed with 2.5% glutaraldehyde, treated with 1% osmium tetroxide, and embedded in epoxy resin. RESULTS: Staining of vessels with CD34 and factor VIII antibodies appeared similar under light microscopy. However, CD34-stained consecutive slices were judged not to reveal vessel structures, and some cells stained with CD45 antibody were similar in appearance to CD34-stained cells. Under CLSM, irregular arrangements of neovascularization, consisting of many branches, were observed, but many positively stained cells not identified as vessels were also seen. Microvessels were distinctly identified under TEM, but the types of individual cells could not be determined. CONCLUSIONS: An integrated, reproducible method for the measurement of MVD is vital. For pancreatic cancer, the "lumen method" is recommended.
机译：背景/目的：大多数恶性肿瘤的微血管密度（MVD）被认为与转移和预后密切相关。通常使用魏德纳的“热点法”来确定MVD，但内皮细胞以外的细胞也可能会被染色。在我们先前的研究中，“热点方法”确定的MVD与预后/转移之间没有相关性。但是，使用“流明法”，我们发现仅与血管结构数量相关。在本研究中，我们使用光学显微镜，共聚焦激光扫描显微镜（CLSM）和透射电子显微镜（TEM）分析了胰腺癌中微血管的染色。方法：在连续切片中通过光学显微镜检查用CD34，VIII因子和CD45抗体对胰腺癌进行微血管染色。对于CLSM，将新鲜切除的标本用VIII因子和异硫氰酸荧光素进行免疫染色。对于TEM，将样品用2.5％的戊二醛固定，用1％的四氧化os处理，然后包埋在环氧树脂中。结果：在光学显微镜下，用CD34和VIII因子抗体对血管的染色似乎相似。然而，判断CD34染色的连续切片不显示血管结构，并且一些用CD45抗体染色的细胞在外观上与CD34染色的细胞相似。在CLSM下，观察到由许多分支组成的新血管形成的不规则排列，但是还观察到许多未鉴定为血管的阳性染色细胞。在TEM下可以清楚地识别微血管，但无法确定单个细胞的类型。结论：一种完整的，可重现的MVD测量方法至关重要。对于胰腺癌，建议使用“腔法”。

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