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首页> 外文期刊>Journal of hepato-biliary-pancreatic surgery >Anti-transforming growth factor-beta1 antibody transiently enhances DNA synthesis during liver regeneration after partial hepatectomy in rats.
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Anti-transforming growth factor-beta1 antibody transiently enhances DNA synthesis during liver regeneration after partial hepatectomy in rats.

机译：抗转化生长因子-β1抗体可在大鼠部分肝切除术后的肝脏再生过程中瞬时增强DNA合成。

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摘要

The regulation of liver regeneration after partial hepatectomy (PHx) is complex and involves many different cytokines. We investigated the role of one of these, transforming growth factor-beta1 (TGF-beta1), an inhibitor of liver regeneration, in a Wistar male rat model, in which anti-TGF-beta1 antibody was injected immediately or 24 h after 70% PHx. Livers from treated animals contained an increased number of cells in S phase, according to 5-bromo-2'-deoxyuridine (BrdU) labeling 36 h after PHx. Antibody administration 24 h after PHx resulted in the highest peak of proliferation; moreover, peak MIB-5 labeling was also observed at that time. However, neither residual liver-weight-to-body-weight ratios nor regeneration rates differed significantly between any of the animals. Therefore, we also measured levels of serum TGF-beta1 and hepatocyte growth factor (HGF; an activator). With antibody administration at 0 or 24 h, TGF-beta1 levels were diminished at 24 or 36 h as compared with levels in control rats, but then rebounded, reaching a delayed peak at 48 or 72 h after PHx, respectively. Interestingly, there were also similar trends in HGF levels. These results indicate that TGF-beta1 may inhibit the G1 checkpoint, and serum TGF-beta1 concentration may influence HGF to regulate liver regeneration and to maintain homeostasis of proliferation after PHx.
机译：肝部分切除术（PHx）后肝脏再生的调节非常复杂，涉及许多不同的细胞因子。我们在Wistar雄性大鼠模型中调查了其中之一转化生长因子β1（TGF-beta1）（肝脏再生抑制剂）的作用，在该模型中，立即或70％后24小时注射抗TGF-beta1抗体PHx。根据PHx后36小时标记的5-溴-2'-脱氧尿苷（BrdU），来自治疗动物的肝脏在S期细胞数量增加。在PHx后24小时施用抗体导致了最高的增殖峰。此外，当时还观察到了MIB-5标记峰。但是，任何动物之间的残余肝重/体重比和再生率均无显着差异。因此，我们还测量了血清TGF-β1和肝细胞生长因子（HGF;一种激活剂）的水平。在0或24 h施用抗体后，与对照组相比，TGF-beta1的水平在24或36 h减少，但随后反弹，分别在PHx后48或72 h达到延迟峰。有趣的是，HGF水平也有类似趋势。这些结果表明，TGF-β1可能会抑制G1检查点，血清TGF-β1的浓度可能会影响HGF调节肝再生并维持PHx后增殖的稳态。

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