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首页> 外文期刊>Journal of Hepatology: The Journal of the European Association for the Study of the Liver >Microwave treatment of serum facilitates detection of hepatitis B virus DNA by the polymerase chain reaction. Results of a study in anti-HBe positive chronic hepatitis B.
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Microwave treatment of serum facilitates detection of hepatitis B virus DNA by the polymerase chain reaction. Results of a study in anti-HBe positive chronic hepatitis B.

机译：血清的微波处理有助于通过聚合酶链反应检测乙型肝炎病毒DNA。抗HBe阳性慢性乙型肝炎的研究结果。

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摘要

Investigation by polymerase chain reaction of HBV-DNA in serum from chronic hepatitis B virus carriers is not widely used for routine diagnosis because polymerase chain reaction assays are complex and may be too sensitive. We investigated the sensitivity, the specificity and the possible value for clinical use of a simplified polymerase chain reaction method in which a single, 30 cycles round of polymerase chain reaction is performed using only 10 microliters of serum treated with microwaves. The efficiency of the polymerase chain reaction in amplifying HBV-DNA was greater after microwave irradiation of serum than after alkaline extraction, but lower than after protein digestion and phenol chloroform precipitation. Despite its simplicity and high sensitivity, the assay was very specific. Studies in anti-HBe positive chronic hepatitis B virus carriers demonstrated HBV-DNA sequences in 1/15 (7%) healthy carriers, in 4/20 (20%) patients with slight alanine aminotransferase elevation, in 16/18 (89%) with marked alanine aminotransferase elevation and in all 20 with fluctuating alanine aminotransferase levels. In the latter, HBV-DNA was detected either at exacerbation (two cases), during remission (one case) or both (17 cases). HBV-DNA was detected by classical dot-blot hybridization in only 24/58 (41%) samples that were positive by the simplified polymerase chain reaction method. Although extremely high sensitivity is not achieved, microwave irradiation of serum simplifies considerably the detection of small amounts of HBV-DNA and makes polymerase chain reaction suitable for monitoring patients in whom weak hepatitis B virus replication is associated with ongoing liver disease.
机译：通过聚合酶链反应对慢性乙型肝炎病毒携带者血清中的HBV-DNA进行研究并未广泛用于常规诊断，因为聚合酶链反应测定法很复杂并且可能过于敏感。我们研究了简化的聚合酶链反应方法的临床应用的敏感性，特异性和可能的​​价值，在该方法中，仅用10微升经微波处理的血清即可进行30个循环的聚合酶链反应。微波辐照血清后，聚合酶链反应扩增HBV-DNA的效率高于碱提取后，但低于蛋白质消化和苯酚氯仿沉淀后。尽管其简单性和高灵敏度，但该检测方法还是非常特异性的。对抗HBe阳性慢性乙型肝炎病毒携带者的研究表明，在1/15（7％）健康携带者中，在4/20（20％）谷丙转氨酶轻微升高的患者中HBV-DNA序列在16/18（89％）中谷丙转氨酶水平明显升高，而所有20种谷丙转氨酶水平却在波动。在后者中，在加重期（2例），缓解期间（1例）或两者（17例）均检测到HBV-DNA。 HBV-DNA仅通过经典的斑点印迹杂交在简化的聚合酶链反应方法中呈阳性的24/58（41％）样品中检测到。尽管没有实现极高的灵敏度，但是血清微波照射大大简化了少量HBV-DNA的检测，并使聚合酶链反应适合于监测乙型肝炎病毒复制弱与持续肝病相关的患者。

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