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首页> 外文期刊>Journal of Immunological Methods >Recycling of ELISA plates for the serological diagnosis of tuberculosis using a Mycobacterium tuberculosis-specific glycolipid antigen.
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Recycling of ELISA plates for the serological diagnosis of tuberculosis using a Mycobacterium tuberculosis-specific glycolipid antigen.

机译：使用结核分枝杆菌特异性糖脂抗原回收ELISA板以进行血清学诊断。

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摘要

A Mycobacterium tuberculosis-specific glycolipid antigen (diacyl-trehalose-DAT) and a battery of defined sera from tuberculous patients were used to evaluate the reliability of recycling ELISA microplates for serodiagnosis of tuberculosis. The reuse of plates was evaluated by the same ELISA technique before and after recycling with an acid buffer to dissociate and release antibodies from antigen-antibody complexes fixed on used plates. The correlation coefficients for comparisons between several new plates used on different days were always higher than r = 0.90, and the variation between tests was always lower than 12%. For comparisons performed systematically between new plates and recycled plates, the values were variable. Up to the fifth recycling, the correlation coefficient was higher than r = 0.60. From the sixth recycling, correlation coefficient values fell to r = 0.381. The results obtained with recycled plates washed manually were much poorer than those obtained after automatic washing. The results described in this report demonstrate that an ELISA test can be performed on recycled plates under the conditions described.
机译：使用结核分枝杆菌特异的糖脂抗原（二酰基海藻糖-DAT）和结核病患者确定的血清，评估回收的ELISA微孔板用于结核病血清诊断的可靠性。在用酸缓冲液再循环以从固定在用过的板上的抗原-抗体复合物中解离和释放抗体之前和之后，通过相同的ELISA技术评估板的再利用。在不同日期使用的几个新板之间进行比较的相关系数始终高于r = 0.90，而测试之间的差异始终低于12％。为了在新板和回收板之间进行系统比较，这些值是可变的。直到第五次循环，相关系数都高于r = 0.60。从第六次循环开始，相关系数值降至r = 0.381。用手动洗涤的回收板获得的结果比自动洗涤后获得的结果差很多。该报告中描述的结果表明，可以在所述条件下对回收的平板进行ELISA试验。

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