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首页> 外文期刊>Journal of Hand Surgery. American Volume >The effect of chondroitinase on nerve regeneration following composite tissue allotransplantation.
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The effect of chondroitinase on nerve regeneration following composite tissue allotransplantation.

机译：软骨素酶对复合组织同种异体移植后神经再生的影响。

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PURPOSE: To improve the degree of functional return and sensibility provided by composite tissue allotransplantation, enhanced nerve regeneration is essential. Chondroitin sulfate proteoglycans are found in the extracellular matrix of nerves and inhibit regenerating axons after injury. Treatment with chondroitinase to remove chondroitin sulfate proteoglycans has been shown to improve nerve regeneration in isolated nerve graft and transection-and-repair models. This study assesses the efficacy of chondroitinase as a neurotherapeutic agent in the setting of composite tissue allotransplantation. METHODS: Adult Lewis rats received either orthotopic hind limb transplants from Brown Norway rat donors (n = 12) or sciatic nerve transection and repair (n = 6). Following approximation of the sciatic nerve, half the animals received intraneural injections of chondroitinase in saline and the other half received intraneural injections of saline alone. Five weeks after transplantation, we killed the animals and analyzed nerves with nonbiased quantitative nerve histomorphometry. One day after transection and repair, we killed animals and harvested sciatic nerves for immunohistochemical staining of cleaved chondroitin sulfate proteoglycans epitope and laminin. We used unpaired t-tests for statistical analysis. RESULTS: Distal to the suture line, chondroitinase-treated animals demonstrated statistically greater total number of fibers and nerve density compared with controls. There were no statistically significant differences in fiber number or nerve density proximal to the suture line or in fiber widths. We observed staining of cleaved chondroitin sulfate proteoglycan epitopes only in treated animals, with no differences observed in the degree of laminin staining. CONCLUSIONS: Intraneural injection of chondroitinase cleaved inhibitory chondroitin sulfate proteoglycans without disrupting proregenerative laminin and resulted in enhanced nerve regeneration after composite tissue allotransplantation. Studies at later time points are needed to assess whether this enhanced nerve regeneration will produce improved functional return.
机译：目的：为提高复合组织同种异体移植提供的功能恢复和敏感性，增强神经再生至关重要。硫酸软骨素蛋白聚糖存在于神经的细胞外基质中，可抑制损伤后的再生轴突。在孤立的神经移植和横切修复模型中，用软骨素酶去除硫酸软骨素蛋白聚糖的治疗已显示可改善神经再生。这项研究评估了软骨素酶作为神经治疗剂在复合组织同种异体移植中的功效。方法：成年Lewis大鼠接受来自Brown Norway大鼠供体的原位后肢移植（n = 12）或坐骨神经横断和修复（n = 6）。坐骨神经逼近后，一半的动物接受神经内注射生理盐水中的软骨素酶，另一半接受单独的神经内注射盐水。移植后五周，我们杀死了动物，并用无偏定量神经组织形态学分析了神经。横切和修复后的一天，我们杀死了动物并收获了坐骨神经，以对硫酸软骨素蛋白多糖表位和层粘连蛋白进行免疫组织化学染色。我们使用未配对的t检验进行统计分析。结果：在缝线的远端，经软骨素酶处理的动物与对照组相比，在统计学上显示出更多的纤维总数和神经密度。缝合线附近的纤维数量或神经密度或纤维宽度均无统计学上的显着差异。我们仅在治疗的动物中观察到裂解的硫酸软骨素蛋白聚糖表位的染色，而层粘连蛋白染色的程度没有差异。结论：神经内注射软骨素酶可切割抑制性硫酸软骨素蛋白聚糖，而不会破坏复合层粘连蛋白，并在复合组织同种异体移植后增强神经再生。需要在以后的时间进行研究，以评估这种增强的神经再生是否会产生改善的功能恢复。

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