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首页> 外文期刊>Biomolecules & therapeutics >Transglutaminase-2 Is Involved in Expression of Osteoprotegerin in MG-63 Osteosarcoma Cells
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Transglutaminase-2 Is Involved in Expression of Osteoprotegerin in MG-63 Osteosarcoma Cells

机译：转谷氨酰胺酶2参与MG-63骨肉瘤细胞中骨保护素的表达。

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Osteoprotegerin (OPG) is a secreted glycoprotein and a member of the tumor necrosis factor receptor superfamily. It usually functions in bone remodeling, by inhibiting osteoclastogenesis through interaction with a receptor activator of the nuclear factor kB (RANKL). Transglutaminases-2 (Tgase-2) is a group of multifunctional enzymes that plays a role in cancer cell metastasis and bone formation. However, relationship between OPG and Tgase-2 is not studied. Therefore, we investigated the involvement of 12-O-Tetradecanoylphorbol 13-acetate in the expression of OPG in MG-63 osteosarcoma cells. Interleukin-ip time-dependently induced OPG and Tgase-2 expression in cell lysates and media of the MG-63 cells by a Western blot. Additional 110 kda band was found in the media of MG-63 cells. 12-O-Tetradecanoylphorbol 13-acetate also induced OPG and Tgase-2 expression. However, an 110 kda band was not found in TPA-treated media of MG-63 cells. Cystamine, a Tgase-2 inhibitor, dose-dependently suppressed the expression of OPG in MG-63 cells. Gene silencing of Tgase-2 also significantly suppressed the expression of OPG in MG-63 cells. Next, we examined whether a band of 110 kda of OPG contains an isopeptide bond, an indication of Tgase-2 action, by monoclonal antibody specific for the isopeptide bond. However, we could not find the isopeptide bond at 110 kda but 77 kda, which is believed to be the band position of Tgase-2. This suggested that 110 kda is not the direct product of Tgase-2's action. All together, OPG and Tgase-2 is induced by IL-1beta or TPA in MG-63 cells and Tgase-2 is involved in OPG expression in MG-63 cells.
机译：骨保护素（OPG）是一种分泌的糖蛋白，是肿瘤坏死因子受体超家族的成员。它通常通过与核因子kB（RANKL）的受体激活剂相互作用来抑制破骨细胞生成，从而在骨骼重塑中发挥作用。 Transglutaminases-2（Tgase-2）是一组多功能酶，在癌细胞转移和骨形成中起作用。但是，尚未研究OPG和Tgase-2之间的关系。因此，我们调查了MG-63骨肉瘤细胞中OPG表达中12-O-十四碳酰佛波醇13-乙酸盐的参与。通过蛋白质印迹，白介素-ip依赖时间诱导了MG-63细胞的细胞裂解液和培养基中的OPG和Tgase-2表达。在MG-63细胞的培养基中发现了另外的110kda带。 12-O-四氢呋喃酚佛波醇13-乙酸盐也诱导OPG和Tgase-2表达。但是，在TPA处理的MG-63细胞培养基中未发现110 kda的条带。半胱胺，一种Tgase-2抑制剂，剂量依赖性地抑制MG-63细胞中OPG的表达。 Tgase-2的基因沉默也显着抑制了MG-63细胞中OPG的表达。接下来，我们通过对异肽键具有特异性的单克隆抗体检查了110 kda的OPG带是否含有异肽键，这是Tgase-2作用的指示。但是，我们找不到在110 kda处而是77 kda处的异肽键，据信这是Tgase-2的条带位置。这表明110 kda不是Tgase-2作用的直接产物。总之，IL-1beta或TPA在MG-63细胞中诱导OPG和Tgase-2，而Tgase-2参与MG-63细胞中OPG的表达。

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