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首页> 外文期刊>Biomolecules & therapeutics >Resveratrol Inhibits Nitric Oxide-Induced Apoptosis via the NF-Kappa B Pathway in Rabbit Articular Chondrocytes
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Resveratrol Inhibits Nitric Oxide-Induced Apoptosis via the NF-Kappa B Pathway in Rabbit Articular Chondrocytes

机译：白藜芦醇通过兔关节软骨细胞中的NF-κB途径抑制一氧化氮诱导的细胞凋亡

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摘要

Resveratrol (trans-3,4'-trihydroxystillbene), a naturally occurring polyphenolic antioxidant found in grapes and red wine, elicits diverse biochemical responses and demonstrates anti-aging, anti-inflammatory, and anti-proliferative effects in several cell types. Previously, resveratrol was shown to regulate differentiation and inflammation in rabbit articular chondrocytes, while the direct production of nitric oxide (NO) in these cells by treatment with the NO donor sodium nitroprusside (SNP) led to apoptosis. In this study, the effect of resveratrol on NO-induced apoptosis in rabbit articular chondrocytes was investigated. Resveratrol dramatically reduced NO-induced apoptosis in chondrocytes, as determined by phase-contrast microscopy, the MTT assay, FACS analysis, and DAPI staining. Treatment with resveratrol inhibited the SNP-induced expression of p53 and p21 and reduced the expression of procaspase-3 in chondrocytes, as detected by western blot analysis. SNP-induced degradation of l-kappa B alpha (hcB-a) was rescued by resveratrol treatment, and the SN50 peptide-mediated inhibition of NF-kappa B (NF-kB) activity potently blocked SNP-induced caspase-3 activation and apoptosis. Our results suggest that resveratrol inhibits NO-induced apoptosis through the NF-kB pathway in articular chondrocytes.
机译：白藜芦醇（trans-3,4'-trihydroxystillbene）是一种天然存在于葡萄和红酒中的多酚抗氧化剂，可引起多种生化反应，并在多种细胞类型中表现出抗衰老，抗炎和抗增殖作用。以前，白藜芦醇显示出调节兔关节软骨细胞的分化和炎症的作用，而通过用NO供体硝普钠（SNP）处理在这些细胞中直接产生一氧化氮（NO）则导致细胞凋亡。在这项研究中，研究了白藜芦醇对NO诱导的兔关节软骨细胞凋亡的影响。通过相差显微镜，MTT分析，FACS分析和DAPI染色确定，白藜芦醇显着减少了NO诱导的软骨细胞凋亡。 Western blot分析表明，白藜芦醇处理可抑制SNP诱导的软骨细胞p53和p21表达，并降低procaspase-3的表达。 SNP诱导的l-kappa Bα（hcB-a）降解通过白藜芦醇治疗得以挽救，并且SN50肽介导的NF-kappa B（NF-kB）活性抑制有效地阻断了SNP诱导的caspase-3激活和凋亡。 。我们的结果表明，白藜芦醇通过关节软骨细胞中的NF-kB途径抑制NO诱导的凋亡。

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