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首页> 外文期刊>Journal of immunoassay >Detection of anti-GM1 ganglioside antibodies in patients with neuropathy by a novel latex agglutination assay.
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Detection of anti-GM1 ganglioside antibodies in patients with neuropathy by a novel latex agglutination assay.

机译：通过新型乳胶凝集测定法检测神经病患者的抗GM1神经节苷脂抗体。

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摘要

Highly elevated titers of serum anti-GM1 ganglioside antibodies are closely associated with multifocal motor neuropathy, but low titers are commonly present in normal individuals or other diseases. Current systems for measuring anti-GM1 antibodies utilize the enzyme-linked immunosorbent assay (ELISA), in which serum dilutions are tested for binding to excess antigen immobilized on the surface of microwells. The ELISA system, however, is relatively time consuming, labor intensive, and costly, in addition to being prone to methodological variability. We have developed a novel agglutination assay for the detection of anti-GM1 antibodies, utilizing GM1 ganglioside-coated latex beads. In contrast to the ELISA system, antibody titers may be quantified by testing for agglutination using latex beads coated with decreasing amounts of antigen. The agglutination assay compares favorably to the ELISA system in sensitivity and specificity, but is considerably less costly and takes only a few minutes to perform.
机译：血清抗GM1神经节苷脂抗体的高滴度与多灶性运动神经病密切相关，但正常个体或其他疾病中通常存在低滴度。当前的测量抗GM1抗体的系统利用酶联免疫吸附测定（ELISA），其中测试血清稀释液与固定在微孔表面的过量抗原的结合。然而，ELISA系统除了易于方法变异之外，还相对耗时，费力且昂贵。我们已经开发了一种新型的凝集测定法，用于利用GM1神经节苷脂涂层的乳胶珠检测抗GM1抗体。与ELISA系统相反，可使用包被有少量抗原的乳胶珠通过测试凝集来定量抗体效价。凝集测定在灵敏度和特异性上均优于ELISA系统，但成本更低，只需几分钟即可完成。

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