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首页> 外文期刊>Journal of industrial and engineering chemistry >Characteristics of menhaden oil ethanolysis by immobilized lipase in supercritical carbon dioxide
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Characteristics of menhaden oil ethanolysis by immobilized lipase in supercritical carbon dioxide

机译：固定化脂肪酶在超临界二氧化碳中的薄荷油乙醇分解特性

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摘要

Ethanolysis of menhaden oil was performed with 1,3-regiospecific lipase to produce diglycerides and monoglycerides containing polyunsaturated fatty acids, and fatty acid ethyl esters. Immobilized lipases like lipozyme TL-IM (Thermomuces lanuginosa immobilized on silica gel) were used for enzymatic ethanolysis. Ethanolysis was carried out in different processes (solvent free, organic solvent and supercritical fluid system) to compare the reaction rate and yield obtained by menhaden oil ethanolysis. Organic solvent (hexane) and supercritical carbon dioxide (SC-CO2) were used as reaction medium. The reaction products were analyzed by gas chromatography (GC), thin layer chromatography (TLC) and high performance liquid chromatography (HPLC). Higher amounts of ethanol as a substrate caused substrate inhibition which dramatically decreased the reaction rate of ethanolysis. To elucidate the effect of pressure, enzymatic ethanolysis was performed in SC-CO2 at pressures ranging from 75 to 121 bar. Enzymatic ethanolysis of menhaden oil in SC-CO2 decreased by substrate inhibition. Reaction rate and optimum amount of ethanol used were depended on SC-CO2 density individually. Kinetic model with substrate inhibition (dead-end inhibition) by excess ethanol was set up to measure the reaction and inhibition rates.
机译：用1，3-区域特异性脂肪酶对薄荷油进行乙醇水解，制得含有多不饱和脂肪酸和脂肪酸乙酯的甘油二酯和甘油单酯。固定化的脂肪酶如脂酶TL-IM（固定在硅胶上的羊毛嗜热菌（Thermomuces lanuginosa）固定化）用于酶促乙醇化。在不同的过程（无溶剂，有机溶剂和超临界流体系统）中进行乙醇分解，以比较薄荷油乙醇分解反应的反应速率和产率。使用有机溶剂（己烷）和超临界二氧化碳（SC-CO2）作为反应介质。通过气相色谱（GC），薄层色谱（TLC）和高效液相色谱（HPLC）分析反应产物。较高含量的乙醇作为底物会导致底物抑制，从而大大降低乙醇分解的反应速率。为了阐明压力的影响，在SC-CO2中于75至121 bar的压力下进行了酶促乙醇水解。底物抑制降低了SC-CO2中薄荷油的酶促乙醇化作用。反应速率和最佳乙醇用量分别取决于SC-CO2密度。建立了具有过量乙醇对底物的抑制（末端抑制）的动力学模型，以测量反应和抑制率。

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