首页> 外文期刊>Journal of Internal Medicine >Cell sheet engineering: a unique nanotechnology for scaffold-free tissue reconstruction with clinical applications in regenerative medicine.
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Cell sheet engineering: a unique nanotechnology for scaffold-free tissue reconstruction with clinical applications in regenerative medicine.

机译:细胞片工程:一种用于无支架组织重建的独特纳米技术,在再生医学中具有临床应用。

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摘要

Cell sheet technology (CST) is based on the use of thermoresponsive polymers, poly(N-isopropylacrylamide) (PIPAAm). The surface of PIPAAms is formulated in such a way as to make its typical thickness <100 nm. In this review, we first focus on how the methods of PIPAAm-grafted surface preparations and functionalization are important to be able to harvest a functional cell sheet, to be further transplanted. Then, we present aspects of tissue mimics and three-dimensional reconstruction of a tissue in vitro. Finally, we give an overview of clinical applications and clinically relevant animal experimentations of the technology, such as cardiomyopathy, visual acuity, periodonty, oesophageal ulcerations and type 1 diabetes.
机译:电池片技术(CST)基于使用热响应性聚合物聚(N-异丙基丙烯酰胺)(PIPAAm)。 PIPAAms的表面以使其典型厚度<100 nm的方式配制。在这篇综述中,我们首先关注于PIPAAm移植的表面制备和功能化方法对于能够收获功能细胞片并进一步移植的重要意义。然后,我们介绍组织模拟和组织的三维重建的方面。最后,我们概述了该技术的临床应用和临床相关的动物实验,例如心肌病,视敏度,牙周病,食道溃疡和1型糖尿病。

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