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首页> 外文期刊>Journal of Lipid Research >Liver and intestinal fatty acid-binding proteins obtain fatty acids from phospholipid membranes by different mechanisms.
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Liver and intestinal fatty acid-binding proteins obtain fatty acids from phospholipid membranes by different mechanisms.

机译：肝和肠的脂肪酸结合蛋白通过不同的机制从磷脂膜获得脂肪酸。

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Intestinal enterocytes contain high concentrations of two cytosolic fatty acid-binding proteins (FABP), liver FABP (L-FABP) and intestinal FABP (I-FABP), which are hypothesized to play a role in cellular fatty acid trafficking. The mechanism(s) by which fatty acids move from membranes to each of these proteins is not known. Here we demonstrate that fluorescent anthroyloxy fatty acid analogues (AOFA) are transferred from phospholipid vesicles to L-FABP versus I-FABP by different mechanisms. For L-FABP a diffusion-mediated transfer process is demonstrated. The AOFA transfer rate from phosphatidylcholine-containing vesicles (POPC) to L-FABP is similar to that observed with another diffusional process, namely inter-membrane AOFA transfer. Furthermore, the AOFA transfer rate was modulated by buffer ionic strength and AOFA solubility, while the transfer rate remained relatively unchanged by the presence of anionic phospholipids in vesicles. In contrast, the data for I-FABP suggest that a transient collisional interaction of I-FABP with the phospholipid membrane occurs during AOFA extraction from the vesicles by the protein. In particular, the presence of the anionic phospholipid cardiolipin in donor vesicles increased the rate of AOFA transfer to I-FABP by 15-fold compared with transfer to POPC vesicles. The effects of ionic strength on transfer suggest that the interaction of I-FABP with cardiolipin-containing vesicles is likely to contain an electrostatic component. Finally, based on the regulation of AOFA transfer to I-FABP compared with transfer from I-FABP, it is hypothesized that apo- and holo-I-FABPs adopt conformations which may differentially promote I-FABP-membrane interactions.In summary, the results suggest that I-FABP, but not L-FABP, can directly extract fatty acids from membranes, supporting the concept that I-FABP may increase the cytosolic flux of fatty acids via intermembrane transfer.
机译：肠道肠上皮细胞含有高浓度的两种胞质脂肪酸结合蛋白（FABP），即肝脏FABP（L-FABP）和肠道FABP（I-FABP），据推测在细胞脂肪酸运输中发挥作用。脂肪酸从膜移动到这些蛋白质中的每一种的机制尚不清楚。在这里，我们证明荧光蒽氧基脂肪酸类似物（AOFA）通过不同的机制从磷脂囊泡转移到L-FABP与I-FABP。对于L-FABP，证明了扩散介导的转移过程。从含磷脂酰胆碱的小泡（POPC）到L-FABP的AOFA转移速率与另一种扩散过程即膜间AOFA转移观察到的速率相似。此外，AOFA转移速率受缓冲液离子强度和AOFA溶解度的调节，而转移速率由于囊泡中存在阴离子磷脂而保持相对不变。相比之下，I-FABP的数据表明I-FABP与磷脂膜的瞬时碰撞相互作用发生在该蛋白质从囊泡中AOFA提取过程中。特别地，在供体囊泡中阴离子磷脂心磷脂的存在与向POPC囊泡的转移相比使AOFA向I-FABP的转移速率增加了15倍。离子强度对转移的影响表明，I-FABP与含心磷脂的囊泡的相互作用可能含有静电成分。最后，基于与从I-FABP转移相比对AOFA转移至I-FABP的调节，假设脱辅基和全I-FABP均采用可能差异性促进I-FABP-膜相互作用的构象。结果表明，I-FABP可以直接从膜中提取脂肪酸，而不是L-FABP，这支持了I-FABP可以通过膜间转移增加脂肪酸胞质通量的概念。

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