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首页> 外文期刊>Journal of Lipid Research >Expression of nitric oxide synthases in subcutaneous adipose tissue of nonobese and obese humans.
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Expression of nitric oxide synthases in subcutaneous adipose tissue of nonobese and obese humans.

机译：一氧化氮合酶在非肥胖和肥胖人的皮下脂肪组织中的表达。

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摘要

Studies have shown evidence of production of nitric oxide (NO) in adipose tissue, as well as inhibition of lipolysis by NO. We have analyzed nitric oxide synthase (NOS) expression in subcutaneous adipose tissue from 13 nonobese and 18 obese male subjects. Using a competitive reverse transcription polymerase chain reaction method, endothelial (eNOS) and inducible (iNOS), but not neuronal (nNOS), nitric oxide synthase mRNA expression was detected in isolated fat cells and pieces of adipose tissue. Tissue mRNA levels for eNOS were 3,814 +/- 825 and 5,956 +/- 476 amol/mg RNA (P = 0.043), and for iNOS 306 +/- 38 and 332 +/- 48 amol/mg RNA, for nonobese and obese individuals, respectively. Western blotting revealed similar eNOS protein levels in isolated fat cells and adipose tissue pieces. Protein levels for eNOS in nonobese and obese individuals, respectively, were (in optical density [OD] units per mm(2) per 100 microgram of total protein) 0.11 +/- 0.08 and 2.80 +/- 1.30 (P = 0.043). iNOS protein was detectable, but not measurable, at low levels in a subset of obese patients (3 of 10). iNOS protein levels could not be detected in nonobese individuals. Hormone-sensitive lipase (HSL), the key regulating enzyme in lipolysis, is reduced in obesity. The expression of HSL protein in subcutaneous adipose tissue was studied in the same subset of patients; in agreement with previous results, HSL levels were reduced in obese subjects: 4.64 +/- 1.10 and 1.27 +/- 0.35 (P = 0.012) in nonobese and obese subjects, respectively.In conclusion, this study shows that eNOS and iNOS, but not nNOS, are present in human subcutaneous adipose tissue. Gene expression and protein levels of eNOS are increased, whereas HSL protein levels are decreased in obesity. It is speculated that increased NO production, preferably by eNOS, and decreased HSL levels may cause decreased subcutaneous adipose tissue lipolysis in obesity. synthases in subcutaneous adipose tissue of nonobese and obese humans.
机译：研究显示了在脂肪组织中产生一氧化氮（NO）以及NO抑制脂解的证据。我们已经分析了来自13名非肥胖和18名肥胖男性受试者皮下脂肪组织中一氧化氮合酶（NOS）的表达。使用竞争性逆转录聚合酶链反应方法，内皮细胞（eNOS）和诱导型（iNOS）而非神经元（nNOS），在分离的脂肪细胞和脂肪组织中检测到一氧化氮合酶mRNA表达。对于非肥胖和肥胖者，eNOS的组织mRNA水平为3,814 +/- 825和5,956 +/- 476 amol / mg RNA（P = 0.043），对于iNOS 306 +/- 38和332 +/- 48 amol / mg RNA个人。蛋白质印迹显示分离的脂肪细胞和脂肪组织碎片中的eNOS蛋白水平相似。非肥胖和肥胖个体中eNOS的蛋白质水平分别为（每100微克总蛋白质每mm（2）每mm（2）的光密度[OD]单位）0.11 +/- 0.08和2.80 +/- 1.30（P = 0.043）。在一部分肥胖患者中，iNOS蛋白水平低但可检测到，但无法测量（10分之3）。在非肥胖个体中无法检测到iNOS蛋白水平。肥胖症中激素敏感性脂肪酶（HSL）是脂解中的关键调节酶。在同一组患者中研究了HSL蛋白在皮下脂肪组织中的表达;与以前的结果一致，肥胖受试者的HSL水平降低：非肥胖受试者和肥胖受试者的HSL水平分别为4.64 +/- 1.10和1.27 +/- 0.35（P = 0.012）。人皮下脂肪组织中存在非nNOS。肥胖中eNOS的基因表达和蛋白质水平升高，而HSL蛋白质水平降低。据推测，优选通过eNOS增加的NO产生和降低的HSL水平可导致肥胖症中皮下脂肪组织脂解的减少。非肥胖和肥胖者皮下脂肪组织中的合酶。

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