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首页> 外文期刊>Journal of Lipid Research >Cholesterol efflux by acute-phase high density lipoprotein: role of lecithin: cholesterol acyltransferase.
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Cholesterol efflux by acute-phase high density lipoprotein: role of lecithin: cholesterol acyltransferase.

机译：急性期高密度脂蛋白引起的胆固醇外流：卵磷脂的作用：胆固醇酰基转移酶。

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HDL plays an initial role in reverse cholesterol transport by mediating cholesterol removal from cells. During infection and inflammation, several changes in HDL composition occur that may affect the function of HDL; therefore, we determined the ability of acute-phase HDL to promote cholesterol removal from cells. Acute-phase HDL was isolated from plasma of Syrian hamsters injected with lipopolysaccharide. Cholesterol removal from J 774 murine macrophages by acute-phase HDL was less efficient than that by control HDL because of both a decrease in cholesterol efflux and an increase in cholesterol influx. LCAT activity of acute-phase HDL was significantly lower than that of control HDL. When LCAT activity of control HDL was inactivated, cholesterol efflux decreased and cholesterol influx increased to the level observed in acute-phase HDL. Inactivation of LCAT had little effect on acute-phase HDL. In GM 3468A human fibroblasts, the ability of acute-phase HDL to remove cholesterol from cells was also lower than that of normal HDL. The impaired cholesterol removal, however, was primarily a result of an increase in cholesterol influx without changes in cholesterol efflux. When control HDL in which LCAT had been inactivated was incubated with fibroblasts, cholesterol influx increased to a level comparable to that of acute-phase HDL, without any change in cholesterol efflux. These results suggest that the ability of acute-phase HDL to mediate cholesterol removal was impaired compared with that of control HDL and the lower LCAT activity in acute-phase HDL may be responsible for this impairment. The decreased ability of acute-phase HDL to remove cholesterol from cells may be one of the mechanisms that account for the well-known relationship between infection/inflammation and atherosclerosis.
机译：HDL通过介导胆固醇从细胞中去除，在逆向胆固醇转运中起着最初的作用。在感染和炎症过程中，HDL组成发生了一些变化，可能会影响HDL的功能。因此，我们确定了急性期HDL促进胆固醇从细胞中去除的能力。从注射了脂多糖的叙利亚仓鼠血浆中分离出急性期HDL。急性期HDL从J 774鼠巨噬细胞中去除胆固醇的效率比对照组HDL差，这是因为胆固醇流出减少和胆固醇流入增加。急性期HDL的LCAT活性显着低于对照HDL。当对照HDL的LCAT活性失活时，胆固醇流出减少，胆固醇流入增加到急性期HDL中观察到的水平。 LCAT的灭活对急性期HDL影响不大。在GM 3468A人成纤维细胞中，急性期HDL从细胞中去除胆固醇的能力也低于正常HDL。然而，胆固醇去除受损主要是由于胆固醇流入增加而胆固醇外排没有改变的结果。当将已灭活LCAT的对照HDL与成纤维细胞一起孵育时，胆固醇的流入增加到与急性期HDL相当的水平，而胆固醇的流出没有任何变化。这些结果表明，与对照HDL相比，急性期HDL介导的胆固醇清除能力受到损害，急性期HDL中较低的LCAT活性可能是造成这种损害的原因。急性期HDL从细胞去除胆固醇的能力下降可能是导致感染/炎症与动脉粥样硬化之间众所周知的关系的机制之一。

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