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首页> 外文期刊>Journal of Korean Forestry Society >Plant Regeneration from Leaf derived Callus of Hybrid Kiwi (Actinidia deliciosa X A. arguta)
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Plant Regeneration from Leaf derived Callus of Hybrid Kiwi (Actinidia deliciosa X A. arguta)

机译：从杂交猕猴桃的叶片衍生愈伤组织中再生植物（猕猴桃X A. arguta）

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Whole plants were regenerated from callus induced from leaf explants in hybrid kiwi (Actinidia deliciosa x A. arguta). Callus was induced from leaf explants which cultured on MS solid medium supplemented with combination of auxin (2,4-D, NAA: 0.1 approx 0.5 mg/1) and cytokinin (BA: 0.1 apporx 0.2 mg/1). Among them, the highest callus formation (96.2%) was obtained from the treatment of 0.5 mg/1 2,4-D+O.l mg/1 NAA+0.05 mg/1 BA. In the experiment of adventitious shoots induction from primary shoots, only a few shoots were produced in the treatment of 1.0 mg/1 BA+0.05 mg/1 IBA or 2.0 mg/1 BA+0.05 mg/1 IBA. As the callus were transferred to the secondary shoot-inducing medium, multiple shoots were obtained from the medium supplemented with 1.0, 2.0 or 5.0 mg/1 zeatin in addition to the mixed treatments of BA, thidiazuron (TDZ) or zeatin. However, no multiple shoots were induced on the BA-contained medium regardless of concentrations. Therefore it turned out that addition of BA to medium was less effective for induction of multiple shoots from callus in Actinidia deliciosa x A. arguta. For producing adventitious roots from shoots, the best frequency of rooting (83.3%) were recorded on the treatment of in vitro rooting (Standardi (St)+1.0 mg/I IBA). Onthe other side, the lowest result (40.0%) were shown in the treatment of 500 mg/1 IBA, 1 hr. Whole plants with shoots and roots were recovered and acclimatized successfully.
机译：从杂交猕猴桃中的叶外植体诱导的愈伤组织中再生出整株植物（Actinidia deliciosa x A. arguta）。由叶片外植体诱导愈伤组织，叶片外植体在补充有生长素（2,4-D，NAA：0.1约0.5 mg / 1）和细胞分裂素（BA：0.1约0.2 mg / 1）的MS固体培养基上培养。其中，从0.5mg / 1，2，4-D + 0.1mg / 1NAA + 0.05mg / 1BA的处理中获得最高的愈伤组织形成（96.2％）。在从初生芽诱导不定芽的实验中，在1.0mg / 1BA + 0.05mg / 1IBA或2.0mg / 1BA + 0.05mg / 1IBA的处理中仅产生了少数芽。将愈伤组织转移至第二芽诱导培养基中，除了BA，噻唑酮（TDZ）或玉米素的混合处理外，还从补充有1.0、2.0或5.0 mg / 1玉米素的培养基中获得了许多芽。但是，无论浓度如何，在含有BA的培养基上都不会诱导出多芽。因此，结果表明，向培养基中添加BA对于诱导猕猴桃x arguta中愈伤组织的多芽的诱导不太有效。为了从芽中产生不定根，在体外生根处理中（Standardi（St）+1.0 mg / IBA）记录了最佳的生根频率（83.3％）。另一方面，在500 mg / 1 IBA处理1小时后，结果最低（40.0％）。具有芽和根的整个植物被成功地回收和驯化。

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