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首页> 外文期刊>Acta Horticulturae >Interspecific Hybridization between Actinidia arguta and A. deliciosa and Embryo Culture In Vitro of Its Fi Hybrids
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Interspecific Hybridization between Actinidia arguta and A. deliciosa and Embryo Culture In Vitro of Its Fi Hybrids

机译：猕猴桃与拟南芥的种间杂交及其Fi杂种的胚培养

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摘要

Interspecific hybidization was achieved beween Actinidia arguta (4x) females and A. deliciosa (6x) males, and hybrid embryos were cultured on a solidified Murashige and Skoog (MS) basal medium, supplemented with 16 different combinations of 3-indoleacetic acid (IAA), 6-benzylaminopurine (BAP) and gibberellins (GA). Best results were obtained when the Fi embryos were cultured with supplementation with l-naphthaleneacetic acid NAA (0.3 mg L~(-1)), BAP (2 mg L~(-1)) and GA (0.2 mg L~(-1)). When the plantlets were cultured in medium with BAP (4 mg L~(-1)) and NAA (0.01 mg L~(-1)) significant improvement in proliferation was achieved within one month. Plantlets were rooted on 1/2 MS medium in combination with different concentrations of indole-3-butyricacid (IBA) or NAA, and high rooting and survival was achieved using 1/2 MS media with NAA (0.2 mg L~(-1)). This study offered a new path for Actinidia breeding.
机译：雌性猕猴桃（4x）和雄性猕猴桃（6x）之间实现种间杂合，并在凝固的Murashige和Skoog（MS）基础培养基上培养杂交胚，并补充16种不同的3-吲哚乙酸（IAA）组合，6-苄氨基嘌呤（BAP）和赤霉素（GA）。当Fi胚胎补充l-萘乙酸NAA（0.3 mg L〜（-1）），BAP（2 mg L〜（-1））和GA（0.2 mg L〜（-1）时，获得了最佳结果。 ））。当小苗在含有BAP（4 mg L〜（-1））和NAA（0.01 mg L〜（-1））的培养基中培养后，在一个月内即可实现增殖的显着改善。将幼苗与不同浓度的吲哚-3-丁酸（IBA）或NAA组合在1/2 MS培养基上生根，使用带有NAA（0.2 mg L〜（-1）的1/2 MS培养基可实现高生根和存活。 ）。该研究为猕猴桃育种提供了新途径。

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