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首页> 外文期刊>Journal of Korean medical science >Expression profiling of calcium induced genes in cultured human keratinocytes.
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Expression profiling of calcium induced genes in cultured human keratinocytes.

机译:钙诱导的基因在培养的人角质形成细胞中的表达谱。

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摘要

Terminal differentiation of skin keratinocytes is a vertically directed multi-step process that is tightly controlled by the sequential expression of a variety of genes. To examine the gene expression profile in calcium-induced keratinocyte differentiation, we constructed a normalized cDNA library using mRNA isolated from these calcium-treated keratinocytes. After sequencing about 10,000 clones, we were able to obtain 4,104 independent genes. They consisted of 3,699 annotated genes and 405 expressed sequence tags (ESTs). Some were the genes involved in constituting epidermal structures and others were unknown genes that are probably associated with keratinocytes. In particular, we were able to identify genes located at the chromosome 1q21, the locus for the epidermal differentiation complex, and 19q13.1, another probable locus for epidermal differentiation-related gene clusters. One EST located at the chromosome 19q13.1 showed increased expression by calcium treatment, suggesting a novel candidate gene relevant to keratinocyte differentiation. These results demonstrate the complexity of the transcriptional profile of keratinocytes, providing important clues on which to base further investigations of the molecular events underlying keratinocyte differentiation.
机译:皮肤角质形成细胞的终末分化是一个垂直定向的多步骤过程,该过程由多种基因的顺序表达严格控制。为了检查钙诱导的角质形成细胞分化中的基因表达谱,我们使用从这些钙处理的角质形成细胞中分离的mRNA构建了标准化的cDNA文库。对大约10,000个克隆进行测序后,我们能够获得4,104个独立基因。它们由3699个带注释的基因和405个表达的序列标签(EST)组成。一些是构成表皮结构的基因,另一些是未知的基因,可能与角质形成细胞有关。特别是,我们能够鉴定位于表皮分化复合物基因座1q21和19q13.1染色体的基因,该基因是与表皮分化相关基因簇的另一个可能基因座。位于染色体19q13.1的一个EST显示通过钙处理增加了表达,这表明与角质形成细胞分化有关的新候选基因。这些结果证明了角质形成细胞转录谱的复杂性,为进一步研究角质形成细胞分化的分子事件提供了重要线索。

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