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首页> 外文期刊>Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society >Histoplasma capsulatum manifests preferential invasion of phagocytic subpopulations in murine lungs.
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Histoplasma capsulatum manifests preferential invasion of phagocytic subpopulations in murine lungs.

机译：荚膜组织胞浆表现出优先侵袭鼠肺中的吞噬细胞亚群。

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Numerous in vitro studies have demonstrated that Histoplasma capsulatum is engulfed by the diverse populations of phagocytic cells including monocytes/macrophages (Mphi), immature dendritic cells (DC), and neutrophils. The in vivo distribution of H. capsulatum has yet to be examined following an intrapulmonary challenge. To accomplish this goal, we engineered GFP into two genetically dissimilar strains of H. capsulatum, G217B and 186R. C57BL/6 mice were infected with each of these strains, and we analyzed the distribution of this fungus in the three major phagocytic populations on successive days. Yeast cells were found in all three populations of cells from Days 1 through 7. Proportionally, DC dominated at Day 1, whereas the majority of yeast cells was detected in neutrophils thereafter. Yeast cells were present in inflammatory and resident Mphi on Day 3, but on Day 7, they were chiefly in inflammatory Mphi. Yeast cells were predominantly in a CD11c(+intermediate/high), F4/80(-), CD11b(+), Ly-6C(+), CD205(+) DC population. Neutralization of TNF-alpha or IFN-gamma produced a significant redistribution of yeast cells. These results reveal the complex nature of intracellular residence of this fungus. Moreover, the findings demonstrate that there is a skewing in the subpopulations of cells that are infected, especially DC.
机译：大量的体外研究表明，荚膜组织胞浆被吞噬细胞的不同种群吞噬，吞噬细胞包括单核细胞/巨噬细胞（Mphi），未成熟树突细胞（DC）和嗜中性粒细胞。肺内攻击后，还需要检查荚膜嗜血杆菌的体内分布。为了实现此目标，我们将GFP工程改造为两种遗传上不同的荚膜梭菌菌株G217B和186R。 C57BL / 6小鼠感染了这些菌株中的每一个，我们连续几天分析了该真菌在三个主要吞噬细胞种群中的分布。从第1天到第7天，在所有三个细胞群中都发现了酵母细胞。成比例地，在第1天DC占主导地位，而此后在嗜中性粒细胞中检测到大多数酵母细胞。在第3天，酵母细胞存在于炎性Mphi中，但在第7天，它们主要存在于炎性Mphi中。酵母细胞主要分布在CD11c（+中/高），F4 / 80（-），CD11b（+），Ly-6C（+），CD205（+）DC种群中。 TNF-α或IFN-γ的中和产生了酵母细胞的大量重新分布。这些结果揭示了这种真菌在细胞内停留的复杂性质。此外，研究结果表明，受感染的细胞（尤其是DC）的亚群存在偏斜。

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