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首页> 外文期刊>Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society >Human epidermal Langerhans cells differ from monocyte-derived Langerhans cells in CD80 expression and in secretion of IL-12 after CD40 cross-linking.
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Human epidermal Langerhans cells differ from monocyte-derived Langerhans cells in CD80 expression and in secretion of IL-12 after CD40 cross-linking.

机译：人表皮朗格汉斯细胞与单核细胞衍生的朗格汉斯细胞在CD80表达和CD40交联后的IL-12分泌方面不同。

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摘要

Langerhans cells (LCs) represent an immature population of myeloid dendritic cells (DCs). As a result of their unique Birbeck granules (BGs), langerin expression, and heterogeneous maturation process, they differ from other immature DCs. Monocyte-derived LCs (MoLCs) mimic epidermal LCs. MoLCs with characteristic BGs are generated by culturing blood-derived monocytes with granulocyte macrophage-colony stimulating factor, interleukin (IL)-4, and transforming growth factor-beta1. Here, we compare maturation-induced antigen expression and cytokine release of LCs with MoLCs. To achieve comparable cell populations, LCs and MoLCs were isolated by CD1c cell sorting, resulting in high purity. In unstimulated cells, CD40 was expressed at equal levels. After stimulation with CD40 ligand (CD40L), LCs and MoLCs acquired CD83 and increased CD86. High CD80 expression was exclusively detected in CD1c-sorted MoLCs. Human leukocyte antigen-DR and CD54 expression was found in all cell populations, however, at different intensities. CD40 triggering increased the potency of LCs and MoLCs to stimulate CD4+ T cell proliferation. Activated MoLCs released IL-12p70 and simultaneously, anti-inflammatory IL-10. The application of the Toll-like receptor ligands peptidoglycan, flagellin, and in particular, lipopolysaccharide (LPS) increased the corelease of these cytokines. LCs secreted IL-10 at a comparable level with MoLCs but failed to produce high amounts of IL-12p70 after application of danger signals. These data indicate that MoLCs as well as LCs display no maturation arrest concerning CD83 and CD86 expression. In difference to MoLCs, LCs resisted activation by CD40L and LPS in terms of IL-12 production. This shows that natural and generated LCs share similar features but differ in relevant functions.
机译：朗格汉斯细胞（LC）代表未成熟的髓样树突状细胞（DC）。由于其独特的Birbeck颗粒（BG），Langerin表达和异构成熟过程，它们不同于其他未成熟的DC。单核细胞衍生的LC（MoLC）模仿表皮LC。具有特征性BG的MoLC是通过将血源性单核细胞与粒细胞巨噬细胞集落刺激因子白介素（IL）-4和转化生长因子β1一起培养而产生的。在这里，我们比较了成熟诱导的抗原表达和LC与MoLC的细胞因子释放。为了获得可比的细胞群，通过CD1c细胞分选分离了LC和MoLC，从而获得了高纯度。在未刺激的细胞中，CD40的表达水平相同。用CD40配体（CD40L）刺激后，LC和MoLC获得CD83，并增加CD86。仅在CD1c分选的MoLC中检测到高CD80表达。在所有细胞群体中均发现了人类白细胞抗原-DR和CD54表达，但强度不同。 CD40触发增加了LC和MoLC刺激CD4 + T细胞增殖的能力。活化的MoLC释放IL-12p70，同时释放抗炎IL-10。 Toll样受体配体肽聚糖，鞭毛蛋白，特别是脂多糖（LPS）的应用增加了这些细胞因子的共释放。 LC分泌的IL-10与MoLC相当，但在施加危险信号后未能产生大量IL-12p70。这些数据表明MoLCs和LCs都没有显示关于CD83和CD86表达的成熟停滞。与MoLC不同，就IL-12产生而言，LC抵抗CD40L和LPS的激活。这表明自然LC和生成的LC具有相似的功能，但相关功能有所不同。

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