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首页> 外文期刊>Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society >Loss of lineage antigens is a common feature of apoptotic lymphocytes.
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Loss of lineage antigens is a common feature of apoptotic lymphocytes.

机译：谱系抗原的丢失是凋亡淋巴细胞的共同特征。

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The analysis of apoptosis in cell populations involves the detection of their specific lineage antigen (LAg) expression. This experimental approach relies on their assumed constant expression, but it is unclear whether such expression is actually maintained during cell death. We examined whether the loss of LAgs is a common feature of apoptotic lymphocytes and whether some might completely lose their LAgs. The changes in the expression of CD3, CD5, CD8, CD4, CD28, CD56, and CD19 were monitored in highly purified lymphocyte populations obtained by negative selection in a fluorescence-activated cell sorter. These were cultured for 24 h with or without phytohemagglutinin or staurosporin. For each LAg-positive subset studied, apoptosis was consistently more common among cells showing partial or total loss of LAg expression compared with cells maintaining their initial LAg levels. The kinetics of expression loss was rapid for CD8, CD56, and CD28, and more than 80% of initial expression was lost in the earlystages of apoptosis but was slower for CD3, CD5, and CD4. For CD3 and CD5, expression was dependent on the apoptotic stimulus used. It is interesting that loss of antigen expression was independent of cell size. This phenomenon was also found in nonmanipulated, highly pure CD19 B lymphocytes of peripheral blood mononuclear cells from B chronic lymphocytic leukemia patients. Loss of LAg expression appeared to be a common feature of apoptotic lymphocytes under all the conditions assayed. The different kinetic patterns of LAg loss suggest apoptotic cells might actively regulate this process.
机译：细胞群体中凋亡的分析涉及检测其特异性谱系抗原（LAg）表达。这种实验方法依赖于它们假定的恒定表达，但是尚不清楚这种表达在细胞死亡期间是否实际上得以维持。我们检查了LAgs的丢失是否是凋亡淋巴细胞的共同特征，以及是否有些人可能会完全丢失其LAgs。在通过荧光激活细胞分选仪中的阴性选择获得的高度纯化的淋巴细胞群体中，监测CD3，CD5，CD8，CD4，CD28，CD56和CD19的表达变化。将它们在有或没有植物血凝素或星形孢菌素的情况下培养24小时。对于研究的每个LAg阳性亚组，与维持其初始LAg水平的细胞相比，凋亡在显示LAg表达部分或全部丧失的细胞中更为普遍。 CD8，CD56和CD28的表达丧失动力学很快，在细胞凋亡的早期阶段，超过80％的初始表达丧失了，而CD3，CD5和CD4则较慢。对于CD3和CD5，表达取决于所用的凋亡刺激。有趣的是，抗原表达的丧失与细胞大小无关。在来自B型慢性淋巴细胞性白血病患者的外周血单核细胞的未经操纵的高纯度CD19 B淋巴细胞中也发现了这种现象。在所有测定的条件下，LAg表达的丧失似乎是凋亡淋巴细胞的共同特征。 LAg丢失的不同动力学模式表明凋亡细胞可能会主动调节这一过程。

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