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首页> 外文期刊>Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society >TNF-related apoptosis-inducing ligand (TRAIL) is expressed throughout myeloid development, resulting in a broad distribution among neutrophil granules.
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TNF-related apoptosis-inducing ligand (TRAIL) is expressed throughout myeloid development, resulting in a broad distribution among neutrophil granules.

机译：TNF相关的凋亡诱导配体（TRAIL）在整个髓样发育过程中表达，导致嗜中性粒细胞颗粒之间广泛分布。

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TRAIL induces apoptosis in a variety of tumor cells. Our laboratory found that human neutrophils contain an intracellular reservoir of prefabricated TRAIL that is released after stimulation with Mycobacterium bovis bacillus Calmette-Guerin. In this study, we examined the subcellular distribution of TRAIL in freshly isolated neutrophils. Neutrophil granules, secretory vesicles (SV), and plasma membrane vesicles were isolated by subcellular fractionation, followed by free-flow electrophoresis, and examined by ELISA and immunoblot. TRAIL was found in all membrane-bound fractions with the highest amounts in the fractions enriched in azurophilic granule (AG) and SV. Immunofluorescence confocal microscopy showed that TRAIL colocalized independently with myeloperoxidase (MPO), lactoferrin (LF), and albumin, respective markers of AG, specific granules, and SV. Furthermore, immunotransmission electron microscopy demonstrated that TRAIL colocalized intracellularly with MPO and albumin. We examined TRAIL expression in PLB-985 cells induced with dimethylformamide and in CD34-positive stem cells treated with G-CSF. Quantitative RT-PCR analysis showed that TRAIL was expressed in each stage of development, whereas MPO and LF were only expressed at distinct times during differentiation. Collectively, these findings suggest that TRAIL is expressed throughout neutrophil development, resulting in a broad distribution among different granule subtypes.
机译：TRAIL诱导多种肿瘤细胞凋亡。我们的实验室发现，人类嗜中性粒细胞含有预制的TRAIL的细胞内储器，该储器在牛分枝杆菌芽孢杆菌Calmette-Guerin刺激后释放。在这项研究中，我们检查了新鲜分离的中性粒细胞中TRAIL的亚细胞分布。中性粒细胞颗粒，分泌囊泡（SV）和质膜囊泡通过亚细胞分级分离，然后自由流动电泳，并通过ELISA和免疫印迹进行检查。在所有膜结合级分中都发现了TRAIL，在富含亲嗜性颗粒（AG）和SV的级分中含量最高。免疫荧光共聚焦显微镜检查显示，TRAIL与髓过氧化物酶（MPO），乳铁蛋白（LF）和白蛋白分别独立地共定位，分别是AG，特定颗粒和SV的标志物。此外，免疫透射电子显微镜证明TRAIL与MPO和白蛋白在细胞内共定位。我们检查了TRAIL在二甲基甲酰胺诱导的PLB-985细胞中以及在用G-CSF处理的CD34阳性干细胞中的表达。定量RT-PCR分析显示TRAIL在发育的每个阶段表达，而MPO和LF仅在分化期间的不同时间表达。总的来说，这些发现表明TRAIL在嗜中性粒细胞的整个发育过程中表达，导致在不同颗粒亚型之间的广泛分布。

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