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首页> 外文期刊>Journal of mass spectrometry: JMS >PSAQ? standards for accurate MS-based quantification of proteins: From the concept to biomedical applications
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PSAQ? standards for accurate MS-based quantification of proteins: From the concept to biomedical applications

机译：PSAQ？准确的基于MS的蛋白质定量分析标准：从概念到生物医学应用

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摘要

Absolute protein quantification, i.e. determining protein concentrations in biological samples, is essential to our understanding of biological and physiopathological phenomena. Protein quantification methods based on the use of antibodies are very effective and widely used. However, over the last ten years, absolute protein quantification by mass spectrometry has attracted considerable interest, particularly for the study of systems biology and as part of biomarker development. This interest is mainly linked to the high multiplexing capacity of MS analysis, and to the availability of stable-isotope-labelled standards for quantification. This article describes the details of how to produce, control the quality and use a specific type of standard: Protein Standard Absolute Quantification (PSAQ?) standards. These standards are whole isotopically labelled proteins, analogues of the proteins to be assayed. PSAQ standards can be added early during sample treatment, thus they can correct for protein losses during sample prefractionation and for incomplete sample digestion. Because of this, quantification of target proteins is very accurate and precise using these standards. To illustrate the advantages of the PSAQ method, and to contribute to the increase in its use, selected applications in the biomedical field are detailed here.
机译：绝对蛋白质定量，即确定生物样品中的蛋白质浓度，对于我们对生物学和生理病理现象的理解至关重要。基于使用抗体的蛋白质定量方法非常有效且被广泛使用。然而，在过去十年中，通过质谱进行绝对蛋白质定量吸引了相当大的兴趣，特别是对于系统生物学的研究以及作为生物标记物开发的一部分。这种兴趣主要与MS分析的高复用能力以及稳定同位素标记的定量标准的可用性有关。本文详细介绍了如何生产，控制质量和使用特定类型的标准品：蛋白质标准品绝对定量（PSAQ？）标准品。这些标准品是完整的同位素标记蛋白，是待分析蛋白的类似物。 PSAQ标准品可以在样品处理的早期添加，因此它们可以校正样品预分离过程中的蛋白质损失和不完全的样品消化。因此，使用这些标准品对目标蛋白进行定量非常准确。为了说明PSAQ方法的优点，并为增加其使用做出贡献，此处详细介绍了生物医学领域中的选定应用。

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