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首页> 外文期刊>Journal of mass spectrometry: JMS >A liquid chromatography-mass spectrometry approach to study 'glucosinoloma' in broccoli sprouts
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A liquid chromatography-mass spectrometry approach to study 'glucosinoloma' in broccoli sprouts

机译：液相色谱-质谱法研究西兰花芽中的“葡萄糖苷瘤”

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摘要

Glucosinolates are an important class of secondary plant metabolites, possessing health-promoting properties. Young broccoli plants are a very good source of glucosinolates with concentrations several times greater than in mature plants. The aim of our study was to develop a liquid chromatography-mass spectrometry and liquid chromatography/tandem mass spectrometry qualitative and quantitative method for the measure of glucosinolates in broccoli sprouts. The described method provides high sensitivity and specificity, allowing a rapid and simultaneous determination of 14 glucosinolates. The proposed method has been validated for eight glucosinolates: glucobrassicin, glucoraphanin, glucoiberin, glucoerucin, progoitrin, gluconapin, sinigrin and glucocheirolin. The linear range was 1-150 μg ml ~(-1), the intra-day and inter-day precision values are within 6% and 8% at the lower limit of quantification, while the overall recovery of the eight glucosinolates was 99 ± 9%. This validated method was used successfully for analysis of glucosinolates content of broccoli sprouts grown in different conditions.
机译：芥子油苷是一类重要的次生植物代谢产物，具有促进健康的特性。年轻的花椰菜植物是硫代葡萄糖苷的很好来源，其浓度是成熟植物中的几倍。我们研究的目的是开发一种用于西兰花芽中芥子油苷含量测定的液相色谱-质谱和液相色谱/串联质谱定性和定量方法。所描述的方法具有很高的灵敏度和特异性，可以快速，同时测定14种芥子油苷。所提出的方法已针对八种芥子油苷进行了验证：葡糖嘧啶，葡糖脂，葡糖素，葡糖苷，普罗戈替林，葡糖那平，芥子苷和葡糖基irolin。线性范围是1-150μgml〜（-1），在定量下限时，日内和日间精度值在6％和8％之内，而八种芥子油苷的总回收率为99± 9％。该验证方法已成功用于分析在不同条件下生长的西兰花芽中的芥子油苷含量。

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