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首页> 外文期刊>Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society >Technical advance: autofluorescence as a tool for myeloid cell analysis.
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Technical advance: autofluorescence as a tool for myeloid cell analysis.

机译：技术进步：自体荧光作为骨髓细胞分析的工具。

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Cellular AF is usually considered a hindrance to flow cytometric analysis. Here, we incorporate AF into analysis of complex mixtures of leukocytes. Using a mouse model, we examined cellular AF at multiple excitation and emission wavelengths, and populations with discrete patterns were gated and examined for surface marker expression. In the spleen, all major myeloid populations were identified. In particular, the approach allowed simultaneous characterization of RPM and resident monocytes. When monocytes and RPM were compared, RPM exhibited a phenotype that was consistent with involvement in physiological processes, including expression of genes involved in lipid and iron metabolism. The presence of large amounts of stored ferric iron within RPM enabled purification of these cells using a magnetic-based approach. When adapted for use on leukocytes isolated from a range of other organs, incorporation of AF into analysis allowed identification and isolation of biologically important myeloid populations, including subsets that were not readily identifiable by conventional cytometric analysis.
机译：通常认为细胞房颤是流式细胞仪分析的障碍。在这里，我们将房颤纳入白细胞复杂混合物的分析中。使用小鼠模型，我们检查了多个激发和发射波长下的细胞房颤，并对具有离散模式的种群进行了门控并检查了表面标记的表达。在脾脏中，鉴定出所有主要的髓样群体。特别地，该方法允许同时表征RPM和驻留单核细胞。当比较单核细胞和RPM时，RPM表现出与参与生理过程一致的表型，包括参与脂质和铁代谢的基因的表达。 RPM中存在大量存储的三价铁，可以使用基于磁性的方法纯化这些细胞。当适用于从一系列其他器官中分离出的白细胞时，将AF整合到分析中可以鉴定和分离生物学上重要的髓样群体，包括常规细胞计数分析不易鉴定的亚群。

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