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Differentiation of Vitis vinifera varieties by MALDI-MS analysis of the grape seed proteins

机译:通过葡萄籽蛋白的MALDI-MS分析区分葡萄

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Until now the study of pathogenic related proteins in grape juice and wine, performed by ESI-MS, LC/ESI-MS, and MALDI/MS, has been proposed for differentiation of varieties. In fact, chitinases and thaumatin-like proteins persist through the vinification process and cause hazes and sediments in bottled wines. An additional instrument, potentially suitable for the grape varieties differentiation, has been developed by MALDI/MS for the grape seed protein analysis. The hydrosoluble protein profiles of seeds extract from three different Vitis vinifera grape (red and white) varieties were analyzed and compared. In order to evaluate the environmental conditions and harvest effects, the seed protein profiles of one grape variety from different locations and harvests were studied.
机译:到目前为止,已经提出了通过ESI-MS,LC / ESI-MS和MALDI / MS进行葡萄汁和葡萄酒中病原相关蛋白的研究,以区分品种。实际上,几丁质酶和类索马甜蛋白在整个酿造过程中持续存在,并在瓶装葡萄酒中引起混浊和沉淀。 MALDI / MS已开发出另一种可能适用于葡萄品种分化的仪器,用于葡萄籽蛋白分析。分析并比较了三种不同葡萄(红葡萄和白葡萄)种子提取物的水溶性蛋白质谱。为了评估环境条件和收成效果,研究了一种来自不同位置和收成的葡萄品种的种子蛋白谱。

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