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首页> 外文期刊>Journal of mass spectrometry: JMS >DART mass spectrometry for rapid screening and quantitative determination of cholesterol in egg pasta
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DART mass spectrometry for rapid screening and quantitative determination of cholesterol in egg pasta

机译：DART质谱用于鸡蛋面食中胆固醇的快速筛选和定量测定

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摘要

To ensure that egg-containing products, such as dried eggs and egg pasta, conform to the technological and legislative requirements for egg content, methods are needed to determine the amount of cholesterol in such products. The conventional approach, direct saponification and hexane extraction followed by cholesterol determination by gas chromatography coupled to a flame ionization detector, is very time consuming. Therefore, we developed a rapid method on the basis of direct analysis in real time coupled to time-of-flight mass spectrometry. Samples were prepared simply by solvent extraction followed by extract filtration. The optimization of certain parameters, including the solvent used and direct analysis in real time ionization gas temperature, had a pronounced effect on the intensities of the produced ions, in particular, the molecular and dehydrated ions of cholesterol and its deuterated analog, cholesterol 2,2,3,4,4,6-d_6, which was used as an internal standard. For the developed method, limits of detection and quantification were 0.03 and 0.05mgg~(-1), respectively. The results of the real samples were compared with those obtained using the conventional approach [limit of detection = 0.002mg g~(-1) and limit of quantification = 0.05mg g~(-1)], and it was found that, although the results obtained using the conventional approach were more accurate, our developed method is much simpler and faster, where the time was dramatically reduced by 87% for executing a screening analysis.
机译：为了确保含蛋的产品（如干蛋和蛋面食）符合蛋含量的技术和法律要求，需要确定这些产品中胆固醇含量的方法。常规方法，直接皂化和己烷萃取，然后通过与火焰离子化检测器耦合的气相色谱法测定胆固醇，非常耗时。因此，我们在实时直接分析和飞行时间质谱的基础上开发了一种快速方法。简单地通过溶剂萃取然后萃取过滤来制备样品。某些参数的优化，包括所用的溶剂和实时电离气体温度的直接分析，对产生的离子强度产生了显着影响，特别是胆固醇及其氘代类似物胆固醇2的分子离子和脱水离子2。 2,3,4,4,6-d_6，用作内部标准。对于开发的方法，检测限和定量限分别为0.03和0.05mgg〜（-1）。将实际样品的结果与使用常规方法获得的结果进行比较[检测极限= 0.002mg g〜（-1），定量极限= 0.05mg g〜（-1）]，发现尽管使用常规方法获得的结果更准确，我们开发的方法更简单，更快捷，执行筛选分析的时间大大减少了87％。

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