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首页> 外文期刊>Journal of mass spectrometry: JMS >Sterol and steroid catabolites from cholesterol produced by the psychrophile Pseudoalteromonas haloplanktis
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Sterol and steroid catabolites from cholesterol produced by the psychrophile Pseudoalteromonas haloplanktis

机译：嗜冷假单胞菌嗜盐菌产生的胆固醇中的甾醇和类固醇分解代谢物

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摘要

Pseudoalteromonas haloplanktis, a psychrotrophilic marine bacterium of biotechnological interest, shows anti-biofilm properties and is particularly relevant for cold storage of vacuum packed seafood. We focused our interest on the activation of cholesterol metabolism in this bacterium as the presence in its genome of a putative 3-ketosteroid-Δ~1-dehydrogenase. This study reports GC-MS and LC-MS/MS profiles of sterols/steroids and their derivatives found in cell extracts of P. haloplanktis grown in a medium with a low content of cholesterol. Here, for the first time, we suggest that P. haloplanktis produces some intermediates of cholesterol catabolism, putatively identified as 24-hydroxycholest-1,4-dien-3-one-26-oic acid, chol-1,4-dien-3-one-24-oic acid, 26-hydroxycholest-4-en-3-one, and pregn-4-en-3-one-20-carboxylic acid, a finding already reported in other microorganisms. The presence of these compounds, also considered steroid precursors, produced by P. haloplanktis in vacuum packed seafood could be of interest for healthy of consumers, as well as, for biotechnological applications in pharmaceutical industry.
机译：嗜盐假单胞菌是一种具有生物技术意义的嗜冷菌海洋细菌，具有抗生物膜特性，特别适用于真空包装海产品的冷藏。我们关注这种细菌中胆固醇代谢的激活，因为其基因组中存在推定的3-酮类固醇-Δ〜1-脱氢酶。这项研究报告了在胆固醇含量低的培养基中生长的嗜盐假单胞菌细胞提取物中发现的甾醇/类固醇及其衍生物的GC-MS和LC-MS / MS谱图。在这里，我们首次建议嗜盐假单胞菌产生胆固醇分解代谢的某些中间体，推测其中间体被确定为24-hydroxycholest-1,4-dien-3-one-26-oic acid，chol-1,4-dien- 3-one-24-oic酸，26-hydroxycholest-4-en-3-one和pregn-4-en-3-one-20-羧酸，其他微生物中已有报道。在真空包装的海产品中，由嗜盐假单胞菌生产的这些化合物（也被视为类固醇前体）可能对健康的消费者以及制药行业的生物技术应用感兴趣。

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