• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of mass spectrometry: JMS >Direct identification of trypanosomatids by matrix-assisted laser desorption ionization-time of flight mass spectrometry (DIT MALDI-TOF MS)
【24h】

Direct identification of trypanosomatids by matrix-assisted laser desorption ionization-time of flight mass spectrometry (DIT MALDI-TOF MS)

机译：通过基质辅助激光解吸电离飞行时间质谱（DIT MALDI-TOF MS）直接鉴定锥虫病

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Accurate and rapid determination of trypanosomatids is essential in epidemiological surveillance and therapeutic studies. Matrix-assisted laser desorption ionization/time of flight mass spectrometry (MALDI-TOF MS) has been shown to be a useful and powerful technique to identify bacteria, fungi, metazoa and human intact cells with applications in clinical settings. Here, we developed and optimized a MALDI-TOF MS method to profile trypanosomatids. trypanosomatid cells were deposited on a MALDI target plate followed by addition of matrix solution. The plate was then subjected to MALDI-TOF MS measurement to create reference mass spectra library and unknown samples were identified by pattern matching using the BioTyper software tool. Several m/z peaks reproducibly and uniquely identified trypanosomatids species showing the potentials of direct identification of trypanosomatids by MALDI-TOF MS. Moreover, this method discriminated different life stages of Trypanosoma cruzi, epimastigote and bloodstream trypomastigote and Trypanosoma brucei, procyclic and bloodstream. T. cruzi Discrete Typing Units (DTUs) were also discriminated in three clades. However, it was not possible to achieve enough resolution and software-assisted identification at the strain level. Overall, this study shows the importance of MALDI-TOF MS for the direct identification of trypanosomatids and opens new avenues for mass spectrometry-based detection of parasites in biofluids. Copyright (C) 2016 John Wiley & Sons, Ltd.
机译：准确快速地测定锥虫病在流行病学监测和治疗研究中至关重要。基质辅助激光解吸电离/飞行时间质谱仪（MALDI-TOF MS）已被证明是一种有用且功能强大的技术，可用于鉴定细菌，真菌，后生动物和人类完整细胞，并应用于临床。在这里，我们开发并优化了MALDI-TOF MS方法来分析锥虫病。将锥虫细胞沉积在MALDI靶板上，然后加入基质溶液。然后将板进行MALDI-TOF MS测量以创建参考质谱库，并使用BioTyper软件工具通过模式匹配来识别未知样品。几个m / z峰可重复且独特地鉴定出锥虫的种类，显示出通过MALDI-TOF MS直接鉴定锥虫的潜力。此外，该方法区分了锥虫，副鞭毛虫和血锥虫和布鲁氏锥虫，顺环血和血锥虫的不同生命阶段。 T. cruzi离散打字单位（DTU）也分为三个分支。但是，无法在应变水平上获得足够的分辨率和软件辅助的识别。总的来说，这项研究表明了MALDI-TOF MS对于直接鉴定锥虫的重要性，并为基于质谱检测生物流体中的寄生虫开辟了新途径。版权所有（C）2016 John Wiley＆Sons，Ltd.

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号