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Physical properties of the probe electrospray ionization (PESI) needle applied to the biological samples

机译:应用于生物样品的探针电喷雾电离(PESI)针的物理特性

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摘要

Probe electrospray ionization (PESI) is a recently developed technique that uses a fine solid needle as a probe for sampling biological materials. In this study, we quantified the volume of liquid sample picked up by the solid needle with the tip diameter of similar to 700 nm and the apex angle of similar to 60 degrees. The amounts of low-viscosity samples (rat urine) loaded on the tip of the needle by a single stroke were 0.35 +/- 0.09 pl. Interestingly, the amount of liquid adhered to the tip did not significantly depend on the protein concentration, but viscosity and surface tension of the sample. Under these conditions, we successfully obtained mass spectra for each biological sample.
机译:探针电喷雾电离(PESI)是一项最近开发的技术,该技术使用细的实心针作为探针对生物材料进行采样。在这项研究中,我们量化了固体针头尖端直径相似于700 nm,顶角相似于60度的液体样品的体积。单次冲程在针尖上加载的低粘度样品(大鼠尿液)量为0.35 +/- 0.09 pl。有趣的是,附着在针尖上的液体量并不明显取决于蛋白质浓度,而是取决于样品的粘度和表面张力。在这些条件下,我们成功获得了每个生物样品的质谱图。

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