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首页> 外文期刊>Journal of mass spectrometry: JMS >LC/ESI-MS/MS characterisation of lipopeptide biosurfactants produced by the Bacillus licheniformis V9T14 strain
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LC/ESI-MS/MS characterisation of lipopeptide biosurfactants produced by the Bacillus licheniformis V9T14 strain

机译：地衣芽孢杆菌V9T14菌株产生的脂肽生物表面活性剂的LC / ESI-MS / MS表征

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摘要

Lipopeptide biosurfactants produced by the Bacillus licheniformis V9T14 strain showed an interesting anti-adhesion activity against biofilm formation of human pathogenic bacterial strains. The chemical characterisation of the crude extract of V9T14 strain was first developed through electrospray ionisationmass spectrometry (ESI-MS) and ESI-MS/MS direct infusions: two sets of molecular ionspeciesbelonging to the fengycin andsurfactin familieswere revealedandtheir structures defined, interpreting their product ion spectra. The LC/ESI-MS analysis of the crude extract allowed to separate in different chromatogram ranges the homologues and the isoforms of the two lipopeptide families. The extract was then fractionated by silica gel chromatography in two main fractions, I and II. The purified biosurfactants were analysed through a new, rapid and suitable LC/ESI-MS/MS method, which allowed characterising the composition and the structures of the produced lipopeptides. LC/ESI-MS/MS analysis of fraction I showed the presence of C_(13), C_(14) and C_(15) surfactin homologues, whose structures were confirmed by the product ion spectra of the sodiated molecules [M + Na]~+ at m/z 1030, 1044 and 1058. LC/ESI-MS/MS analysis of fraction II confirmed the presence of two main fengycin isoforms, with the protonated molecules [M + H]~+ at m/z 1478 and 1506 corresponding to C_(17) fengycin A and C_(17) fengycin B, respectively. Other homologues (C_(14) to C_(16)) were revealed and confirmed as belonging to fengycin A or B according to the retention times and the product ions generated, althoughwith the same nominalmass. Finally, a relative percentage content of each homologue for both lipopeptides families in the whole extract was proposed.
机译：地衣芽孢杆菌V9T14菌株产生的脂肽生物表面活性剂对人病原菌菌株的生物膜形成表现出有趣的抗粘附活性。首先通过电喷雾电离质谱（ESI-MS）和ESI-MS / MS直接输注技术开发了V9T14菌株粗提物的化学特性：揭示了两组属于丰霉素和表面活性素的分子离子种类，并定义了它们的结构，解释了它们的产物离子光谱。允许在不同色谱图中分离的粗提液的LC / ESI-MS分析范围为两个脂肽家族的同系物和同工型。然后将提取物通过硅胶色谱分离为两个主要馏分I和II。通过一种新的，快速且合适的LC / ESI-MS / MS方法对纯化的生物表面活性剂进行了分析，从而可以表征所产生的脂肽的组成和结构。 LC / ESI-MS / MS对馏分I的分析表明存在C_（13），C_（14）和C_（15）表面活性蛋白同系物，其结构已通过固体分子[M + Na]的产物离子光谱确认。在m / z 1030、1044和1058时为〜+。级分II的LC / ESI-MS / MS分析证实存在两种主要的风霉素同工型，质子化分子[M + H]〜+在m / z 1478和1506。分别对应于C_（17）fengycin A和C_（17）fengycinB。根据保留时间和产生的产物离子，揭示了其他同系物（C_（14）至C_（16）），并确认其属于丰霉素A或B，尽管其标称质量相同。最后，提出了整个提取物中两个脂肽家族的每个同源物的相对百分比含量。

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