• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of mass spectrometry: JMS >Identification of carbonylation sites in apomyoglobin after exposure to 4-hydroxy-2-nonenal by solid-phase enrichment and liquid chromatography-electrospray ionization tandem mass spectrometry
【24h】

Identification of carbonylation sites in apomyoglobin after exposure to 4-hydroxy-2-nonenal by solid-phase enrichment and liquid chromatography-electrospray ionization tandem mass spectrometry

机译：固相富集-液相色谱-电喷雾串联质谱法鉴定暴露于4-羟基-2-壬烯醛后的血红蛋白中羰基化位点

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Identification of protein carbonylation because of covalent attachment of a lipid peroxidation end-product was performed by combining proteolytic digestion followed by solid-phase hydrazide enrichment and liquid chromatography (LC)-electrospray ionization (ESI) tandem mass spectrometry (MS/MS) using both collision-induced dissociation ((ID) and electron capture dissociation (ECD). To evaluate this approach, we selected apomyoglobin and 4-hydroxy-2-nonenal (4-HNE) as a model protein and a representative end-product of lipid peroxidation, respectively. Although the characteristic elimination of 4-HNE (156 Da) in CID was found to serve as a signature tag for the modified peptides, generation of nearly complete fragment ion series because of efficient peptide backbone cleavage (in most cases over 75%) and the capability to retain the labile 4-HNE moiety of the tryptic peptides significantly aided the elucidation of primary structural information and assignment of exact carbonylation sites in the protein, when ECD was employed. We have concluded that solid-phase enrichment with both CID- and ECD-MS/MS are advantageous during an in-depth interrogation and unequivocal localization of 4-HNE-induced carbonylation of apomyoglobin that occurs via Michael addition to its histidine residues.
机译：通过将蛋白水解消化，固相酰肼富集和液相色谱（LC）-电喷雾电离（ESI）串联质谱（MS / MS）结合使用，对由于脂质过氧化终产物的共价连接而引起的蛋白质羰基化进行鉴定为了评估这种方法，我们选择了apglooglobin和4-hydroxy-2-nonenal（4-HNE）作为模型蛋白和脂质过氧化的代表性终产物，以评估这种方法。碰撞诱导解离（（ID）和电子捕获解离（ECD）。尽管发现CID中4-HNE（156 Da）的特征消除可作为修饰肽的标记，但由于有效的肽主链裂解，生成了几乎完整的片段离子系列（大多数情况下超过75％） ）和保留胰蛋白酶肽不稳定的4-HNE部分的能力大大有助于阐明主要结构信息并确定确切的羰基化位点当使用ECD时，蛋白质中的蛋白质含量降低。我们已经得出结论，在深入研究和4-HNE诱导的apomyoglobin羰基化的羰基化的深入研究和明确定位过程中，固相富集在CID-和ECD-MS / MS上是有利的，这是由于Michael加成至其组氨酸残基而引起的。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号