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首页> 外文期刊>Journal of mass spectrometry: JMS >Detection of hazelnut oil in extra-virgin olive oil by analysis of polar components bymicro-solid phase extraction based on hydrophilic liquid chromatography and MALDI-ToF mass spectrometry
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Detection of hazelnut oil in extra-virgin olive oil by analysis of polar components bymicro-solid phase extraction based on hydrophilic liquid chromatography and MALDI-ToF mass spectrometry

机译：通过亲水液相色谱和MALDI-ToF质谱微固相萃取分析极性成分来检测初榨橄榄油中的榛子油

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摘要

The oil polar fraction may have a great potential for the characterization of vegetable oils and for the individuation of adulterations. In particular, adulteration of extra-virgin olive oil (EVOO) with hazelnut oil (HO) is one of the most difficult ones to detect due to the similar composition as regards triacylglycerol, total sterol and fatty acid profile. A new micro-solid phase extraction (μ-SPE) procedure based on hydrophilic liquid chromatography (HILIC) micro-columns was developed for the selective extraction and enrichment of polar compounds from EVOO and HO before matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-ToF-MS) analysis. The method permits a simple and fast qualitative analysis of the polar fraction of the oils under study; furthermore, somepeaks (such as the m/z ions 496.39, 520.46 and 522.47) were found tobe present only in HO, indicating that they could be diagnostic for the presence of HO in EVOO. In order to verify the potential of the method for the individuation of this adulteration, EVOO was progressively adulterated with variable quantities of HO, subjected to the HILIC enrichment and finally to MALDI-ToF-MS analysis; the detection of adulteration was possible up to the level of 5%. Eventually, diagnostic polar compounds were identified as lysophosphatidylcholine (LPC) (16 : 0/0 : 0), LPC (18 : 2/0 : 0), LPC (18 : 1/0 : 0) by means of capillary liquid chromatography-electrospray ionization-quadrupole-ToF-MS (CapLC-ESI-Q-ToF-MS) analysis.
机译：极性油分在植物油的表征和掺假的个性化方面可能具有很大的潜力。特别是，由于三酰基甘油，总固醇和脂肪酸谱的组成相似，因此特级初榨橄榄油（EVOO）与榛子油（HO）的掺假是最难检测的一种。开发了一种基于亲水液相色谱（HILIC）微柱的新型微固相萃取（μ-SPE）方法，用于在基质辅助激光解吸电离时间之前从EVOO和HO中选择性萃取和富集极性化合物。飞行质谱（MALDI-ToF-MS）分析。该方法可以对所研究油类的极性组分进行简单，快速的定性分析。此外，发现某些峰（例如m / z离子496.39、520.46和522.47）仅存在于HO中，这表明它们可以诊断EVOO中HO的存在。为了验证这种掺假方法的潜力，用可变数量的HO逐步掺入EVOO，进行HILIC富集，最后进行MALDI-ToF-MS分析。掺假率最高可达5％。最终，通过毛细管液相色谱法将诊断性极性化合物鉴定为溶血磷脂酰胆碱（LPC）（16：0/0：0），LPC（18：2/0：0 0），LPC（18：1/0：0 0）-电喷雾电离四极杆ToF-MS（CapLC-ESI-Q-ToF-MS）分析。

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